Graduate Thesis Or Dissertation
 

The stability of pelargonidin-based anthocyanins in natural and model systems

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  • Pelargonidin 3-glucoside (pgd 3-glu), pelargonidin 3-sophoroside (pgd 3-soph), and pelargonidin 3-sophoroside 5-glucoside acylated with cinnamic and malonic acids (acyl-pgd 3-soph 5-glu) were extracted from strawberries (Fragaria anannassa cv, Totem), nasturtium flowers (Tropaeolum majus), and radish peel (Raphanus sativus L. cv, fuego), respectively. Their stability was studied in natural and model systems. Natural systems consisted of strawberry juice at 8 °brix and strawberry concentrate at 65 °brix that were spiked with the anthocyanins (ACNS) to double the initial pigment concentration. Model systems at low, intermediate, and high water activity levels consisted of pH 3.4 citrate buffer, glycerol, and pigment. Changes in pigment, degradation index, color, and relative peak area were monitored during storage in the dark at 25°C. Ascorbic acid degradation was also monitored in the natural systems. Anthocyanin (ACN) degradation followed first order kinetics. No difference in stability of the samples was found with fortified pgd derivatives; however, there was significant difference in the degradation of ACNS between natural and model systems. The half life (t [subscript 1/2]) of the ACNS ranged from 3.5 to 5 days in the concentrate, from 8 to 12 days in juice, and from 58 to 934 days in model systems. In general, high Aw increased ACN degradation. Ascorbic acid degradation followed first order kinetics and was accompanied by ACN degradation.
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