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Measuring the Natural Fluorescence of Phytoplankton Cultures

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dc.creator Laney, S. R.
dc.creator Letelier, R. M.
dc.creator Desiderio, R. A.
dc.creator Abbott, Mark R.
dc.creator Kiefer, D. A.
dc.creator Booth, C. R.
dc.date.accessioned 2012-02-20T19:20:57Z
dc.date.available 2012-02-20T19:20:57Z
dc.date.issued 2001-11
dc.identifier.citation Laney, S. R., R. M. Letelier, R. A. Desiderio, M. R. Abbott, D. A. Kiefer, C. R. Booth, 2001: Measuring the Natural Fluorescence of Phytoplankton Cultures. Journal of Atmospheric and Oceanic Technology, 18, 1924–1934. en_US
dc.identifier.uri http://hdl.handle.net/1957/27849
dc.description.abstract A laboratory instrument, the Natural Fluorescence Chemostat, was developed to measure the natural fluorescence of phytoplankton cultures. With this instrument, the physical and chemical environment of a culture can be manipulated with respect to temperature, pH, nutrient delivery rate, and light intensity, while the natural fluorescence and a weak stimulated fluorescence are continuously recorded with high temporal resolution. The geometry and spectral distribution of the artificial light field minimize the contribution of scattering to the natural fluorescence signal. Preliminary investigations with the marine diatom T. weissflogii (Bacillariophyceae) indicate that the instrument can detect natural fluorescence signals in broadband artificial light fields as bright as 1250 μmol quanta m⁻² s⁻¹. Since the influence of environmental factors on natural fluorescence is not well understood, laboratory experiments are essential for investigating how ocean physics and chemistry influence this signal. This instrument provides a quantitative means to examine how the magnitude and kinetics of phytoplankton natural fluorescence vary in response to changes in the physical and chemical environment. en_US
dc.description.sponsorship This research was supported by NASA (Contract NAS5-31360) as was the original construction of the prototype (Contract NAS7-969). en_US
dc.language.iso en_US en_US
dc.publisher American Meteorological Society en_US
dc.relation.ispartofseries Journal of Atmospheric and Oceanic Technology en_US
dc.relation.ispartofseries Vol. 18 no. 11. en_US
dc.title Measuring the Natural Fluorescence of Phytoplankton Cultures en_US
dc.type Article en_US
dc.identifier.doi 10.1175/1520-0426(2001)018<1924:MTNFOP>2.0.CO;2


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