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Evaluation of a potential Chylamydia psittaci vaccine candidate using recombinant vaccinia virus encoding the infection-specific C. psittaci GPIC proteins IncA and TroA

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dc.contributor.advisor Rockey, Daniel D.
dc.contributor.advisor Hruby, Dennis E.
dc.creator Werth, Eric P.
dc.date.accessioned 2012-04-18T17:29:27Z
dc.date.available 2012-04-18T17:29:27Z
dc.date.copyright 2001-06-27
dc.date.issued 2001-06-27
dc.identifier.uri http://hdl.handle.net/1957/28803
dc.description Graduation date: 2002 en_US
dc.description.abstract Members of the family Chlamydiae cause a wide range of diseases. Chlamydia trachomatis and C. pneumoniae are most commonly associated with human disease. C. psittaci and C. pecorum are largely animal pathogens, although C. psittaci can cause pneumonia in the elderly and immunocompromised. A vaccine against these pathogens is desirable, but although multiple vaccine regimens have been examined, none have proven truly effective. Studies were conducted to evaluate the use of recombinant vaccinia virus (VV) vectors encoding chlamydial proteins as vaccine candidates using a guinea pig model. In the first study, guinea pigs were immunized with varying amounts of attenuated VV encoding either the M6 protein of Streptococcus pyogenes or chloramphenicol acetyl transferase (CAT) from E. coli. The purpose of this study was: (1) determine how much attenuated virus can be given intranasally to guinea pigs without causing death; (2) characterize the humoral and secretory antibody response to both the viral vector and M6 protein; and (3) develop a protocol for animal manipulation, and sample collection and storage for use in future research. The results obtained indicate that 10⁹ PFU of attenuated VV can be given intranasally to guinea pigs. Serum IgG was detected against VV proteins, as determined by immunoblotting. Antibodies against M6 could not be similarly detected in serum, or by direct enzyme linked immunosorbant assay (ELISA). IgG could not be detected by immunoblotting against either VV or M6 in saliva. The purpose of the second part of this research was to evaluate the potential efficacy of a vaccine using the C. psittaci guinea pig inclusion conjunctivitis (GPIC) strain proteins IncA and TroA. Guinea pigs were immunized intranasally with either PBS, control vaccinia virus, rVV:IncA, or rVV:TroA. Three weeks after immunization animals were challenged by ocular infection with C. psittaci elementary bodies (EBs). Eye swabs were taken following challenge and titered to determine the chlamydial load. Results indicate that rVV:TroA provides no protection against chlamydial challenge. Titers from rVV:IncA immunized animals appeared to be somewhat lower than those of the controls on day 4 post-challenge. This difference, however, proved not to be statistically significant. A single immunization with rVV:IncA or rVV:TroA was thus shown not lead to a protective immune response in guinea pigs under the conditions tested. en_US
dc.language.iso en_US en_US
dc.subject.lcsh Chlamydia en_US
dc.subject.lcsh DNA vaccines en_US
dc.subject.lcsh Vaccinia en_US
dc.subject.lcsh Bacterial vaccines en_US
dc.title Evaluation of a potential Chylamydia psittaci vaccine candidate using recombinant vaccinia virus encoding the infection-specific C. psittaci GPIC proteins IncA and TroA en_US
dc.type Thesis/Dissertation en_US
dc.degree.name Master of Science (M.S.) in Microbiology en_US
dc.degree.level Master's en_US
dc.degree.discipline Science en_US
dc.degree.grantor Oregon State University en_US
dc.contributor.committeemember Ream, Walter
dc.contributor.committeemember Tornquist, Susan
dc.description.digitization File scanned at 300 ppi (Monochrome, 256 Grayscale, 24-bit Color) using Capture Perfect 3.0.82 on a Canon DR-9080C in PDF format. CVista PdfCompressor 4.0 was used for pdf compression and textual OCR. en_US
dc.description.peerreview no en_us


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