Undergraduate Thesis Or Project
 

Removal of Unreacted Dinitrophenyl Hydrazine from Carbonyl Derivatives

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https://ir.library.oregonstate.edu/concern/undergraduate_thesis_or_projects/dj52w6222

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  • OSU/HHMI Summer Undergraduate Research Symposium Presentation.
  • Small molecule metabolomics is a promising new field in medicine. Studying the chemical effects of individual cellular processes is a powerful approach in determining physiological impacts of various factors such as age, cancer stage, and genetic variability at a cellular level. However, the reactive nature and small size of many molecules impedes both data collection and analysis. One common complication is the loss of reactive small molecules due to ex vivo participation in spurious side reactions during the preparation of samples for analyses such as mass spectrometry. To prevent these spurious side reactions, carbonyl compounds must first be derivatized with an agent such as dinitrophenyl hydrazine (DNPH). However, following the derivatization, excess DNPH complicates mass spectrometry results; therefore, it must be removed. The purpose of this study is to determine a method by which carbonyl compounds harvested from tissues can be derivatized with DNPH to form hydrazones that are then separated from the excess DNPH and resolubulized in a solvent compatible withmass spectrometry analysis. The development of a general method by which carbonyls harvested from tissues can be derivatized and analyzed with mass spectrometry will facilitate the study of many carbonyl-compound metabolomic pathways.
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file details Removal of Unreacted Dinitrophenyl Hydrazine from Carbonyl Derivatives-Barkal.pptx 07/21/2017 Public
file details Removal of Unreacted Dinitrophenyl Hydrazine from Carbonyl Derivatives-Barkal.pdf 07/21/2017 Public