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DNA Mismatch Repair-Dependent Suppression of Genotoxicity of Benzo[a]pyrene Diol Epoxide.

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dc.contributor.advisor Buermeyer, Andrew
dc.creator Kernan, Casey
dc.date.accessioned 2012-06-19T17:05:24Z
dc.date.available 2012-06-19T17:05:24Z
dc.date.copyright 2012-05-25
dc.date.issued 2012-06-19
dc.identifier.uri http://hdl.handle.net/1957/29963
dc.description June 2012 en_US
dc.description.abstract Abstract DNA mismatch repair (MMR) plays an important role in preserving genomic stability and reducing cancer risk. Environmental exposures to polycyclic aromatic hydrocarbons (PAHs) are believed to contribute significantly to carcinogenesis. PAHs are found in food, air, water and soil and upon bioactivation can form diol epoxides which are electrophilic in nature and can adduct to DNA, creating bulky PAH-DNA lesions. Preliminary data in the Buermeyer laboratory demonstrated that mutations induced by benzo[a]pyrene diol epoxide (BPDE) occur at higher rates in MLH1-deficient HCT 116+Ch2 cell line (30±7 mutations per 100 nM BPDE) versus a genetically matched MLH1-proficient line HCT 116+Ch3 cell line (10±4 mutations per 100 nM BPDE). I hypothesized that the role for MMR in suppressing mutation is a phenomenon generalizable to other cell lines. I measured BPDE-induced mutation rates in the parent cell line HCT 116, and in another genetically matched MSH6-proficient and -deficient set. I found an induced mutation rate of 30±5 mutations per 100 nM BPDE using HCT 116 cells, not significantly different from the induced mutation rate with HCT 116+Ch2 cells. In addition, we characterized mutants from HCT 116+Ch2 clones and identified 25 individual mutations with the predominance of G→T transversions. , en_US
dc.language.iso en_US en_US
dc.subject DNA Mismatch Repair en_US
dc.subject Polycyclic Aromatic Hydrocarbons en_US
dc.subject Cancer en_US
dc.title DNA Mismatch Repair-Dependent Suppression of Genotoxicity of Benzo[a]pyrene Diol Epoxide. en_US
dc.type Research Paper en_US
dc.degree.level Bachelor's en_US
dc.degree.discipline Agricultural Sciences en_US
dc.degree.grantor Oregon State University en_US
dc.contributor.committeemember Hays, John B.
dc.contributor.committeemember Field, Katharine G.

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