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The putative protein methyltransferase LAE1 controls cellulase gene expression in Trichoderma reesei

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dc.creator Seiboth, Bernhard
dc.creator Karimi, Razieh Aghcheh
dc.creator Phatale, Pallavi A.
dc.creator Linke, Rita
dc.creator Hartl, Lukas
dc.creator Sauer, Dominik G.
dc.creator Smith, Kristina M.
dc.creator Baker, Scott E.
dc.creator Freitag, Michael
dc.creator Kubicek, Christian P.
dc.date.accessioned 2012-07-03T18:06:52Z
dc.date.available 2012-07-03T18:06:52Z
dc.date.issued 2012-06
dc.identifier.citation Seiboth, B., Karimi, R. A., Phatale, P. A., Linke, R., Hartl, L., Sauer, D. G., Smith, K. M., Baker, S. E., Freitag, M. and Kubicek, C. P. (2012), The putative protein methyltransferase LAE1 controls cellulase gene expression in Trichoderma reesei. Molecular Microbiology, 84: 1150–1164. doi: 10.1111/j.1365-2958.2012.08083.x en_US
dc.identifier.uri http://hdl.handle.net/1957/30493
dc.description This is the publisher’s final pdf. The published article is copyrighted by Wiley-Blackwell and can be found at: http://onlinelibrary.wiley.com/journal/10.1111/(ISSN)1365-2958/issues. To the best of our knowledge, one or more authors of this paper were federal employees when contributing to this work. en_US
dc.description.abstract Trichoderma reesei is an industrial producer of enzymes that degrade lignocellulosic polysaccharides to soluble monomers, which can be fermented to biofuels. Here we show that the expression of genes for lignocellulose degradation are controlled by the orthologous T. reesei protein methyltransferase LAE1. In a lae1 deletion mutant we observed a complete loss of expression of all seven cellulases, auxiliary factors for cellulose degradation, β-glucosidases and xylanases were no longer expressed. Conversely, enhanced expression of lae1 resulted in significantly increased cellulase gene transcription. Lae1-modulated cellulase gene expression was dependent on the function of the general cellulase regulator XYR1, but also xyr1 expression was LAE1-dependent. LAE1 was also essential for conidiation of T. reesei. Chromatin immunoprecipitation followed by high-throughput sequencing (‘ChIP-seq’) showed that lae1 expression was not obviously correlated with H3K4 di- or trimethylation (indicative of active transcription) or H3K9 trimethylation (typical for heterochromatin regions) in CAZyme coding regions, suggesting that LAE1 does not affect CAZyme gene expression by directly modulating H3K4 or H3K9 methylation. Our data demonstrate that the putative protein methyltransferase LAE1 is essential for cellulase gene expression in T. reesei through mechanisms that remain to be identified. en_US
dc.description.sponsorship This study was supported by a grant of the Austrian Science Foundation to C.P.K. (P 21266), funds from the OSU Computational and Genome Biology Initiative and PNNL to M.F. K.M.S. was supported by funds from an NIH grant (P01GM068087). en_US
dc.language.iso en_US en_US
dc.publisher Wiley-Blackwell en_US
dc.relation.ispartofseries Molecular Microbiology en_US
dc.relation.ispartofseries Vol. 84 no. 6 en_US
dc.title The putative protein methyltransferase LAE1 controls cellulase gene expression in Trichoderma reesei en_US
dc.type Article en_US
dc.identifier.doi 10.1111/j.1365-2958.2012.08083.x

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