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Accelerated Identification of Proteins by Mass Spectrometry by Employing Covalent Pre-Gel Staining with Uniblue A

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dc.creator Mata-Gomez, Marco A.
dc.creator Yasui, Matthew T.
dc.creator Guerrero-Rangel, Armando
dc.creator Valdes-Rodrıguez, Silvia
dc.creator Winkler, Robert
dc.date.accessioned 2012-07-30T17:56:34Z
dc.date.available 2012-07-30T17:56:34Z
dc.date.issued 2012-02-17
dc.identifier.citation Mata-Gómez, M. A., Yasui, M. T., Guerrero-Rangel, A., Valdés-Rodríguez, S., & Winkler, R. (2012). Accelerated identification of proteins by mass spectrometry by employing covalent pre-gel staining with uniblue A. PloS One, 7 (2), e31438. en_US
dc.identifier.uri http://hdl.handle.net/1957/31649
dc.description This is the publisher’s final pdf. The published article is copyrighted by PLOS and can be found at: www.plosone.org. en_US
dc.description.abstract Background: The identification of proteins by mass spectrometry is a standard method in biopharmaceutical quality control and biochemical research. Prior to identification by mass spectrometry, proteins are usually pre-separated by electrophoresis. However, current protein staining and de-staining protocols are tedious and time consuming, and therefore prolong the sample preparation time for mass spectrometry. Methodology and Principal Findings: We developed a 1-minute covalent pre-gel staining protocol for proteins, which does not require de-staining before the mass spectrometry analysis. We investigated the electrophoretic properties of derivatized proteins and peptides and studied their behavior in mass spectrometry. Further, we elucidated the preferred reaction of proteins with Uniblue A and demonstrate the integration of the peptide derivatization into typical informatics tools. Conclusions and Significance: The Uniblue A staining method drastically speeds up the sample preparation for the mass spectrometry based identification of proteins. The application of this chemo-proteomic strategy will be advantageous for routine quality control of proteins and for time-critical tasks in protein analysis. en_US
dc.language.iso en_US en_US
dc.publisher PLoS One en_US
dc.relation.ispartofseries PloS One en_US
dc.relation.ispartofseries Vol. 7 no. 2 en_US
dc.subject Polyacrylamide gels en_US
dc.subject Statistifcal model en_US
dc.subject Electrophoresis en_US
dc.subject Proteomics en_US
dc.subject Digestion en_US
dc.subject Search en_US
dc.subject Silver en_US
dc.subject Sensitivity en_US
dc.subject Generation en_US
dc.subject Resistance en_US
dc.title Accelerated Identification of Proteins by Mass Spectrometry by Employing Covalent Pre-Gel Staining with Uniblue A en_US
dc.type Article en_US

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