Article
 

Comparison of Meldola’s Blue Staining and Hatching Assay with Potato Root Diffusate for Assessment of Globodera sp. Egg Viability

Public Deposited

Downloadable Content

Download PDF
https://ir.library.oregonstate.edu/concern/articles/xd07gt577

Descriptions

Attribute NameValues
Creator
Abstract
  • Laboratory-based methods to test egg viability include staining with Meldola's Blue and/or juvenile (J2) hatching assays using potato root diffusate (PRD). These two methods have not been tested under identical conditions to directly compare their assessments of Globodera egg viability. Using two bioassay strategies, cysts from a Glabodera sp. population found in Oregon were subjected to both viability assessment methods. In strategy one, intact cysts were first stained with Meldola's Blue (primary staining) and eggs were then transferred to PRD (secondary hatching). In the second strategy, intact cysts were exposed to PRD (primary hatching) and then unhatched eggs were transferred to Meldola's Blue (secondary staining). Two different cohorts of cysts were evaluated using these experimental strategies: cohort I was comprised Of cysts produced on potato in the greenhouse that exhibited low hatch when exposed to PRD and cohort 2 consisted of field-collected cysts whose eggs yielded significant hatch when exposed to PRD Percentage viability was calculated and is expressed as the number of hatched J2 or unstained eggs/total number of eggs within a cyst. With field-produced cysts, primary staining with Meldola's Blue and hatching with PRD produced similar viability estimates, with averages of 74.9% and 76.3%, respectively. In contrast, with greenhouse-produced cysts the two methods yielded much lower and unequal estimates 32.4% to 2.2%, respectively for primary hatching and staining methods. In addition, J2 hatch from Unstained (viable) greenhouse-produced eggs was 13.7% after secondary exposure to PRD compared to 61.5% for field-produced eggs. The majority of eggs remaining unhatched after primary exposure to PRD (> 87%) stained with Meldola's Blue regardless of cyst cohort. Staining with Meldola's Blue provided a conservative assessment of egg viability compared to hatch assay with PRD regardless of diapause.
  • Keywords: egg hatch, juvenile, Globodera, Meldola’s Blue, method, potato root diffusate, cyst nematode
Resource Type
Date Available
Date Issued
Citation
  • Kroese, D., Zasada, I., & Ingham, R. (2011). Comparison of meldola's blue staining and hatching assay with potato root diffusate for assessment of globodera sp egg viability. JOURNAL OF NEMATOLOGY, 43(3-4), 182-186.
Journal Title
Journal Volume
  • 43
Journal Issue/Number
  • 43528
Academic Affiliation
Rights Statement
Publisher
Language
Replaces

Relationships

Parents:

This work has no parents.

Items

Thumbnail Title Date Uploaded Visibility Actions
file details KroeseDuncanBotanyPlantPathologyComparisonMeldola_sBlue.pdf 2017-07-10 Public Download