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The mitochondrial genomes of Euphausia pacifica and Thysanoessa raschii sequenced using 454 next-generation sequencing, with a phylogenetic analysis of their position in the Malacostracan family tree

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dc.creator Johansson, Mattias L.
dc.creator Sremba, Angela L.
dc.creator Feinberg, Leah R.
dc.creator Banks, Michael A.
dc.creator Peterson, William T.
dc.date.accessioned 2012-09-05T20:29:22Z
dc.date.available 2012-09-05T20:29:22Z
dc.date.issued 2012-09
dc.identifier.citation Johansson, M., Sremba, A., Feinberg, L., Banks, M., & Peterson, W. (2012). The mitochondrial genomes of euphausia pacifica and thysanoessa raschii sequenced using 454 next-generation sequencing, with a phylogenetic analysis of their position in the malacostracan family tree. MOLECULAR BIOLOGY REPORTS, 39(9), 9009-9021. doi: 10.1007/s11033-012-1772-z en_US
dc.identifier.uri http://hdl.handle.net/1957/33236
dc.description This is the publisher’s final pdf. The published article is copyrighted by Springer and can be found at: http://www.springer.com/life+sciences/animal+sciences/journal/11033. To the best of our knowledge, one or more authors of this paper were federal employees when contributing to this work. en_US
dc.description.abstract Euphausiid krill play a critical role in coastal and oceanic food webs, linking primary producers to upper trophic levels. In addition, some species support commercial fisheries worldwide. Despite their ecological importance, the genetics of these important species remain poorly described. To improve our understanding of the genetics of these ecological links, we sequenced the mitochondrial genomes of two species of North Pacific krill, Euphausia pacifica and Thysanoessa raschii, using long-range PCR and 454 GS Junior next-generation sequencing technology. The E. pacifica mitogenome (14,692 + base pairs (bp)) encodes 13 protein-coding genes (PCGs), two ribosomal RNA (rRNA) genes, and at least 22 transfer RNA (tRNA) genes. The T. raschii mitogenome (14,240 + bp) encodes 13 PCGs, two rRNA genes, and at least 19 tRNA genes. The gene order in both species is similar to that of E. superba. Comparisons between Bering Sea and Yellow Sea E. pacifica revealed a total of 644 variable sites. The most variable protein-coding gene were atp8 (7.55 %, 12 of 159 sites variable), nad4 (6.35 %, 85 variable sites) and nad6 (6.32 %, 33 variable sites). Phylogenetic analyses to assess the phylogenetic position of the Euphausiacea, using the concatenated nucleic acid sequences of E. pacifica and T. raschii along with 46 previously published malacostracan mitogenomes, support the monophyly of the order Decapoda and indicate that the Euphausiacea share a common ancestor with the Decapoda. Future research should utilize this sequence data to explore the population genetics and molecular ecology of these species. en_US
dc.description.sponsorship This work was supported by a NOAA award to CIMRS (Award No. NA06NMF4550286). en_US
dc.language.iso en_US en_US
dc.publisher Springer en_US
dc.relation.ispartofseries Molecular Biology Reports en_US
dc.relation.ispartofseries Vol. 39 no. 9 en_US
dc.subject mtDNA en_US
dc.subject Mitochondrial genome en_US
dc.subject Phylogeny en_US
dc.subject Malacostraca en_US
dc.subject Euphausiacea en_US
dc.title The mitochondrial genomes of Euphausia pacifica and Thysanoessa raschii sequenced using 454 next-generation sequencing, with a phylogenetic analysis of their position in the Malacostracan family tree en_US
dc.type Article en_US
dc.description.peerreview yes en_US
dc.identifier.doi 10.1007/s11033-012-1772-z


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