Graduate Thesis Or Dissertation

 

Exopolysaccharide biosynthesis by a natural lactococcal ropy isolate Public Deposited

Downloadable Content

Download PDF
https://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/j38609386

Descriptions

Attribute NameValues
Creator
Abstract
  • The genes coding for the expression of a ropy exopolysaccharide responsible for commercially desirable textural and rhealogical traits in fermented milk products by a natural lactococcal ropy isolate were sought. Using a transposon mutagenesis vector, pGh9:ISS1, three mutants lacking expression of the ropy exopolysaccharide were isolated. One of the mutants was chosen for further characterization. Using a Southern hybridization analysis, the interrupted gene was localized to the chromosome. The non-ropy mutant was further characterized and shown to be unable to produce ropy exopolysaccharide in fermented milk. A 2006 by fragment of the interrupted gene was sequenced. The DNA sequence over a short region showed homology to sugar transfer enzymes found in exopolysaccharide biosynthesis pathways. The DNA sequence was translated into its predicted amino acid sequences and two partial open reading frames of 236 and 338 amino acid residues in length were identified. These open reading frames were found to exhibit identity to glycosyltransferases present in exopolysaccharide biosynthesis pathways in other bacteria.
Resource Type
Date Available
Date Issued
Degree Level
Degree Name
Degree Field
Degree Grantor
Commencement Year
Advisor
Academic Affiliation
Non-Academic Affiliation
Subject
Rights Statement
Publisher
Peer Reviewed
Language
Digitization Specifications
  • File scanned at 300 ppi (Monochrome, 8-bit Grayscale, 24-bit Color) using ScandAll PRO 1.8.1 on a Fi-6670 in PDF format. CVista PdfCompressor 4.0 was used for pdf compression and textual OCR.
Replaces

Relationships

Parents:

This work has no parents.

In Collection:

Items