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Characterization of the Na⁺-transporting NADH:ubiquinone oxidoreductase (NQR) in Vibrio cholerae

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dc.creator Fassio, Sara R.
dc.creator Minato, Yusuke
dc.creator Quinn, Matthew J.
dc.creator Faulkner, Wyatt J.
dc.creator Hase, Claudia C.
dc.date.accessioned 2012-09-19T23:17:13Z
dc.date.available 2012-09-19T23:17:13Z
dc.date.issued 2012
dc.identifier.uri http://hdl.handle.net/1957/33670
dc.description.abstract We previously reported that inhibition of the Na⁺ translocating NADH:ubiquinone oxidoreductase (NQR), either by chemical inhibition or mutation, increased toxT transcription in Vibrio cholerae. In this study, we revealed that the nqr mutant strain showed similar phenotypes as the Escherichia coli NADH dehydrogenase I (nuo) mutant strain (e.g. growth defect after the mid log growth phase and higher acetic acid production). The increased growth and toxT expression in the nqr mutant relative to the wild type strain appears to be growth phase dependent. However, after longer growth, nqr showed lower amounts of cholera toxin production compared to the parent strain. Interestingly, the nqr mutant strain showed a similar level of toxT expression in the presence of L-lactate, which is known to stimulate respiration. Through Biolog® Phenotype Microarray (PM) analysis, we found that the V. cholerae nqr mutant strain had defects in a broad spectrum of metabolism functions, including amino acid, carboxylic acid, phosphorus, and sulfur utilization, indicating an important role of NQR in V. cholerae metabolism. In addition, nqr mutation increased osmotic sensitivity in V. cholerae. Some of the defects identified by PM analysis, including NaCl sensitivity, were restored by the addition of L-lactate. en_US
dc.description.sponsorship OSU Undergraduate Research, Innovation, Scholarship & Creativity (URISC); OSU Howard Hughes Medical Institute (HHMI) Undergraduate Summer program en_US
dc.language.iso en_US en_US
dc.subject Vibrio cholerae en_US
dc.subject Cholera en_US
dc.subject Biolog en_US
dc.subject ToxT en_US
dc.title Characterization of the Na⁺-transporting NADH:ubiquinone oxidoreductase (NQR) in Vibrio cholerae en_US
dc.title.alternative Characterization of the Sodium-Translocating NADH-Ubiquinone Oxidoreductase (NQR) on Vibrio cholerae Metabolism and Virulence Gene Regulation en_US
dc.type Poster en_US
dc.description.digitization Microsoft PowerPoint presentation (.pptx). Slide dimensions: 56" x 36" en_US
dc.description.peerreview no en_US


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