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Bacterial 16S ribosomal DNA analysis of pyrrolizidine alkaloid detoxifying enrichments from the ovine rumen

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dc.contributor.advisor Craig, A. Morrie
dc.creator Gray, Diane R.
dc.date.accessioned 2012-10-08T18:39:05Z
dc.date.available 2012-10-08T18:39:05Z
dc.date.copyright 1998-02-05
dc.date.issued 1998-02-05
dc.identifier.uri http://hdl.handle.net/1957/34218
dc.description Graduation date: 1998 en_US
dc.description.abstract Bacterial cultures enriched from sheep rumen fluid have demonstrated the ability to detoxify pyrrolizidine alkaloids (seneciphylline and jacobine) in tansy ragwort (Senecio jacobaea). The microbes are difficult to isolate using classical anaerobic techniques, therefore, microbes from two different enrichment cultures demonstrating similar degradation activity were identified using their 16S ribosomal RNA genes. Gene sequences from a rich medium enrichment were matched to Clostridium bifermentans, Prevotella ruminicola, Escherichia coif, and from a minimal medium enrichment to, C. clostridiiforme, C. aminophilum, Streptococcus bovis, and Butyrivibrio fibrosolvens. There were no identical organisms between the two libraries, but the common genus was Clostridium. en_US
dc.language.iso en_US en_US
dc.subject.lcsh Pyrrolizidines -- Toxicology en_US
dc.subject.lcsh Tansy ragwort -- Toxicology en_US
dc.subject.lcsh Alkaloids -- Toxicology en_US
dc.title Bacterial 16S ribosomal DNA analysis of pyrrolizidine alkaloid detoxifying enrichments from the ovine rumen en_US
dc.type Thesis/Dissertation en_US
dc.degree.name Master of Science (M.S.) in Microbiology en_US
dc.degree.level Master's en_US
dc.degree.discipline Science en_US
dc.degree.grantor Oregon State University en_US
dc.description.digitization File scanned at 300 ppi (Monochrome, 256 Grayscale) using Capture Perfect 3.0 on a Canon DR-9050C in PDF format. CVista PdfCompressor 4.0 was used for pdf compression and textual OCR. en_US
dc.description.peerreview no en_us


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