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Calicivirus recombinant expressing fusion protein reacting to multiple calicivirus typing sera

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dc.contributor.advisor Smith, Alvin W.
dc.creator Stone, Michael A.
dc.date.accessioned 2012-10-09T21:48:58Z
dc.date.available 2012-10-09T21:48:58Z
dc.date.copyright 1996-07-31
dc.date.issued 1996-07-31
dc.identifier.uri http://hdl.handle.net/1957/34288
dc.description Graduation date: 1997 en_US
dc.description.abstract The Caliciviridae contains many viruses which are pathogenic to humans, marine mammals, domestic animals, and numerous species of wildlife. Currently there is no single assay to detect antigenic response to the multiple serotypes of calicivirus. The development of calicivirus specific synthetic peptides having highly conserved epitopes common to many serotypes would facilitate the development of a simple and rapid serologic assay for calicivirus antibodies irrespective of the serotype. Calicivirus cDNA recombinants which express fusion proteins that react with multiple calicivirus typing sera may be useful in the development of a serologic assay for antibodies to caliciviruses. For this purpose RNA was isolated from cell culture infected with San Miguel sea lion virus type 5 (SMSV-5) and used to construct a cDNA library, named SMSV-5 lambda. Immunoassay techniques were used to screen the SMSV-5 lambda library and a second cDNA library, named SMSV-5RT, also constructed from SMSV-5. One recombinant named 8-SN was identified which produced a fusion protein that reacted positively with a pool of four polyclonal calicivirus typing sera (SMSV-5, SMSV-13, SMSV-15, and SMSV-17). This construct was amplified, induced, and a fusion protein identified which reacted positively in four western blot assays using individual polyclonal typing sera to the caliciviruses SMSV-13, SMSV-15, SMSV-16, and SMSV-17. en_US
dc.language.iso en_US en_US
dc.subject.lcsh Caliciviruses en_US
dc.title Calicivirus recombinant expressing fusion protein reacting to multiple calicivirus typing sera en_US
dc.type Thesis/Dissertation en_US
dc.degree.name Master of Science (M.S.) in Veterinary Science en_US
dc.degree.level Master's en_US
dc.degree.discipline Veterinary Medicine en_US
dc.degree.grantor Oregon State University en_US
dc.contributor.committeemember Matsumoto, Masakazu
dc.contributor.committeemember Wolff, Jerry
dc.description.digitization File scanned at 300 ppi (Monochrome) using Capture Perfect 3.0.82 on a Canon DR-9080C in PDF format. CVista PdfCompressor 4.0 was used for pdf compression and textual OCR. en_US
dc.description.peerreview no en_us


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