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Differential decay of human faecal Bacteroides in marine and freshwater

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dc.creator Green, Hyatt C.
dc.creator Shanks, Orin C.
dc.creator Sivaganesan, Mano
dc.creator Haugland, Richard A.
dc.creator Field, Katharine G.
dc.date.accessioned 2012-11-05T23:35:23Z
dc.date.available 2012-11-05T23:35:23Z
dc.date.issued 2011-12
dc.identifier.citation Green, H. C., Shanks, O. C., Sivaganesan, M., Haugland, R. A., & Field, K. G. (2011). Differential decay of human faecal bacteroides in marine and freshwater. Environmental Microbiology, 13(12), 3235-3249. doi: 10.1111/j.1462-2920.2011.02549.x en_US
dc.identifier.uri http://hdl.handle.net/1957/34884
dc.description This is the publisher’s final pdf. The published article is copyrighted by Society for Applied Microbiology and can be found at: http://www.sfam.org.uk/. en_US
dc.description.abstract Genetic markers from Bacteroides and other faecal bacteria are being tested for inclusion in regulations to quantify aquatic faecal contamination and estimate public health risk. For the method to be used quantitatively across environments, persistence and decay of markers must be understood. We measured concentrations of contaminant molecular markers targeting Enterococcus and Bacteroides spp. in marine and freshwater microcosms spiked with human sewage and exposed to either sunlight or dark treatments. We used Bayesian statistics with a delayed Chick-Watson model to estimate kinetic parameters for target decay. DNA-and RNA-based targets decayed at approximately the same rate. Molecular markers persisted (could be detected) longer in marine water. Sunlight increased the decay rates of cultured indicators more than those of molecular markers; sunlight also limited persistence of molecular markers. Within each treatment, Bacteroides markers had similar decay profiles, but some Bacteroides markers significantly differed in decay rates. The role of extracellular DNA in persistence appeared unimportant in the microcosms. Because conditions were controlled, microcosms allowed the effects of specific environmental variables on marker persistence and decay to be measured. While marker decay profiles in more complex environments would be expected to vary from those observed here, the differences we measured suggest that water matrix is an important factor affecting quantitative source tracking and microbial risk assessment applications. en_US
dc.description.sponsorship The US Environmental Protection Agency (USEPA), through its Office of Research and Development, partially funded and collaborated in the research. Continued support from Department of Microbiology, Oregon State University was invaluable. en_US
dc.language.iso en_US en_US
dc.publisher Society for Applied Microbiology en_US
dc.relation.ispartofseries Environmental Microbiology en_US
dc.relation.ispartofseries Vol. 13 no. 12 en_US
dc.subject 16S ribosomal RNA en_US
dc.subject Real time PCR en_US
dc.subject Quantitative PCR en_US
dc.subject Genetic markers en_US
dc.subject Sunlight inactivation en_US
dc.subject Propidium monoazide en_US
dc.subject Growth rate en_US
dc.subject Microbial ecology en_US
dc.subject Bayesian method en_US
dc.subject Bacteria en_US
dc.title Differential decay of human faecal Bacteroides in marine and freshwater en_US
dc.type Article en_US
dc.description.peerreview yes en_US
dc.identifier.doi 10.1111/j.1462-2920.2011.02549.x


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