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Effects of stress on the reproductive performance and physiology of rainbow trout (Oncorhynchus mykiss)

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dc.contributor.advisor Schreck, Carl B.
dc.contributor.advisor Fitzpatrick, Martin S.
dc.creator Contreras Sánches, Wilfrido M.
dc.date.accessioned 2012-11-07T19:41:13Z
dc.date.available 2012-11-07T19:41:13Z
dc.date.copyright 1995-10-24
dc.date.issued 1995-10-24
dc.identifier.uri http://hdl.handle.net/1957/34905
dc.description Graduation date: 1996 en_US
dc.description.abstract The environment under which fish are maintained as broodstock before reproduction is often stressful; however, the impact of stress on broodstock and gamete quality is not well known. We investigated the effects of stress over the final stages (i.e. the 3 months preceding ovulation) of sexual maturation of female rainbow trout, Oncorhynchus mykiss, on their reproductive performance and physiology and that of their progeny. Stress was administered over the period of early vitellogenesis (one and a half months), late vitellogenesis-final maturation (one and a half months), or during both periods (three months). Each stress treatment and control was triplicated with eight females in each replicate (n=24 fish per treatment). The eggs and progeny of each female were kept separate and observations made for four months after transfer to rearing tanks. Cortisol levels were measured in plasma, ovarian fluid and eggs by radioimmunoassay. Fish that experienced stress during final maturation and those that were under stress during the whole experiment spawned on average two weeks earlier than the control group. In contrast, fish stressed during the period of early vitellogenesis spawned at the same time as the controls. Absolute fecundity and fertilization were not significantly affected in any treatment group; however, significant differences were found in relative fecundity. Stress applied early in vitellogenesis resulted in smaller eggs and swim-up fry; but, these differences were not found in juveniles 8 weeks after hatching. Furthermore, we found no differences in survival of the progeny or resistance to the fish pathogen Vibrio anguillarum. Circulating levels of cortisol were high at ovulation in all groups, but significantly less cortisol was observed in the ovarian fluid and eggs. Sex hormone concentrations were high in plasma; however, they were several orders of magnitude lower in the ovarian fluid. These differences were not as extreme as those observed for cortisol. Lower levels of cortisol and sex steroids in ovarian fluid and eggs compared to that which is available from plasma suggests that there is a mechanism by which the female protects the eggs from potentially deleterious effects of prolonged exposure to elevated concentrations of steroids. en_US
dc.language.iso en_US en_US
dc.subject.lcsh Rainbow trout -- Reproduction en_US
dc.subject.lcsh Rainbow trout -- Effect of stress on en_US
dc.subject.lcsh Rainbow trout -- Fertility en_US
dc.title Effects of stress on the reproductive performance and physiology of rainbow trout (Oncorhynchus mykiss) en_US
dc.type Thesis/Dissertation en_US
dc.degree.name Master of Science (M.S.) in Fisheries Science en_US
dc.degree.level Master's en_US
dc.degree.discipline Agricultural Sciences en_US
dc.degree.grantor Oregon State University en_US
dc.description.digitization File scanned at 300 ppi (Monochrome, 8-bit Grayscale) using ScandAll PRO 1.8.1 on a Fi-6670 in PDF format. CVista PdfCompressor 4.0 was used for pdf compression and textual OCR. en_US
dc.description.peerreview no en_us


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