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Distribution of Potato virus Y Strains in Tubers during the Post-Harvest Period

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https://ir.library.oregonstate.edu/concern/articles/dr26xz037

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  • Russet Burbank, Russet Norkotah, and Shepody tubers infected with Potato virus Y strains (PVYO, PVYN:O, PVYNTN) were tested from storage at 4A degrees C at the initiation of sprouting and then the same tubers were tested again from storage at 4A degrees C seventy eight days later. Samples were taken from eyes in the stem, middle, and bud (distal end) areas of the tubers. Testing of the samples was done with an enzyme-linked immunosorbent assay (ELISA) and negative samples were retested using reverse transcription-polymerase chain reactions (RT-PCR). The ELISA alone and the ELISA+PCR combined results were evaluated for each of the strains, sample locations, sample time, and cultivar. Results show that ELISA underestimated the actual percentage of tubers with virus. When test results were combined to show a more accurate percentage, PVYN:O was unevenly distributed in some cultivars, but PVYO and PVYNTN were evenly distributed 78 days after initial sprouting. Results show that protocols for PVY post-harvest testing used by state seed certification agencies should be written to specify the amount of time needed before sampling and that specific protocols may be needed based on cultivar in order to accurately detect PVY in tuber samples.
  • This is the publisher’s final pdf. The published article is copyrighted by Springer and can be found at: http://www.springer.com/?SGWID=0-102-0-0-0
  • Keywords: PVY, ELISA, RT-PCR, Virus detection
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  • Whitworth, J. L., Hamm, P. B., & Nolte, P. (2012). Distribution of potato virus Y strains in tubers during the post-harvest period. American Journal of Potato Research, 89(2), 136-141. doi: 10.1007/s12230-012-9235-6
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  • 89
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  • 2
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  • This work was partially funded by the USDA/ CREES Special Potato Grant Program.
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