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In Vivo Quantification Reveals Extensive Natural Variation in Mitochondrial Form and Function in Caenorhabditis briggsae

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https://ir.library.oregonstate.edu/concern/articles/bc386k30s

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  • In We have analyzed natural variation in mitochondrial form and function among a set of Caenorhabditis briggsae isolates known to harbor mitochondrial DNA structural variation in the form of a heteroplasmic nad5 gene deletion (nad5 Delta) that correlates negatively with organismal fitness. We performed in vivo quantification of 24 mitochondrial phenotypes including reactive oxygen species level, membrane potential, and aspects of organelle morphology, and observed significant amongisolate variation in 18 traits. Although several mitochondrial phenotypes were non-linearly associated with nad5D levels, most of the among-isolate phenotypic variation could be accounted for by phylogeographic clade membership. In particular, isolate-specific mitochondrial membrane potential was an excellent predictor of clade membership. We interpret this result in light of recent evidence for local adaptation to temperature in C. briggsae. Analysis of mitochondrial-nuclear hybrid strains provided support for both mtDNA and nuclear genetic variation as drivers of natural mitochondrial phenotype variation. This study demonstrates that multicellular eukaryotic species are capable of extensive natural variation in organellar phenotypes and highlights the potential of integrating evolutionary and cell biology perspectives.
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  • Hicks KA, Howe DK, Leung A, Denver DR, Estes S (2012) In Vivo Quantification Reveals Extensive Natural Variation in Mitochondrial Form and Function in Caenorhabditis briggsae. PLoS ONE 7(8): e43837. doi:10.1371/journal.pone.0043837
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  • 7
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  • 8
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  • This work was funded by National Institutes of Health grant 5 R01 GM087628-02 to DRD and SE, PSU Faculty Enhancement grant to SE, and an American Heart Association Predoctoral Fellowship 11PRE4880069 to KAH. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
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