Article

 

A Real-Time PCR Assay for Early Detection of Eastern Filbert Blight Public Deposited

Downloadable Content

Download PDF
https://ir.library.oregonstate.edu/concern/articles/3484zh66s

Descriptions

Attribute NameValues
Creator
Abstract
  • Eastern filbert blight (EFB) is a devastating disease of European hazelnut, Corylus avellana, which causes economic losses in Oregon, where 99% of the U.S. crop is produced. The causal fungus, Anisogramma anomala, is native to eastern North America, where it is found associated with the American hazelnut (C. americana). Although C. americana is tolerant, EFB causes cankers, branch dieback, and death of C. avellana. Detection and identification of A. anomala is time consuming using conventional methods because the fungus can only be cultured from sporulating perithecia and the disease symptoms and signs only show 12 to 16 months after infection. In this study, a TaqMan real-time polymerase chain reaction (PCR) assay based on a ribosomal DNA internal transcribed spacer was developed for A. anomala. The assay was validated with multiple isolates of A. anomala, closely related species, common environmental microorganisms, and over 100 C. avellana samples. The real-time PCR assay detected as low as 0.12 pg of A. anomala genomic DNA, and positively diagnosed EFB on 82% of asymptomatic plants as early as 15 weeks from infection. The real-time PCR assay is more sensitive and faster than traditional diagnostic methods. It can facilitate hazelnut breeding and disease management by early and accurate diagnosis of EFB.
  • Keywords: European hazelnut, Oregon, Pacific Northwest, Resistance, Markers, Inoculation, Anisogramma anomala, New Jersey, Corylus avellana, Accessions
Resource Type
DOI
Date Available
Date Issued
Citation
  • Molnar, T., Walsh, E., Capik, J., Sathuvalli, V., Mehlenbacher, S., Rossman, A., & Zhang, N. (2013). A real-time PCR assay for early detection of eastern filbert blight. Plant Disease, 97(6), 813-818. doi:10.1094/PDIS-11-12-1041-RE
Journal Title
Journal Volume
  • 97
Journal Issue/Number
  • 6
Academic Affiliation
Rights Statement
Funding Statement (additional comments about funding)
  • This work was funded by the New Jersey Agricultural Experiment Station, the Rutgers Center for Turfgrass Science, Hatch funds provided by the United States Department of Agriculture (USDA)-NIFA, and the USDA-NIFA Specialty Crops Research Initiative Competitive Grant 2009-51181-06028.
Publisher
Peer Reviewed
Language
Replaces

Relationships

Parents:

This work has no parents.

Items