Graduate Thesis Or Dissertation
 

Factors affecting labeling efficiency of technetium-99m to red blood cells

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  • An in vitro labeling procedure was developed based on optimal conditions of anticoagulant, stannous ion concentration, incubation time, and incubation temperature. This procedure was used to investigate the effects of various agents on Tc-99m RBC labeling yields. These agents were inorganic ions, therapeutic agents, biochemicals, chelating agents, oxidizing agents, an enzyme poison, and methemoglobinemia agents. The experiments for most of the agents consisted of both post- and pretinning procedures. For post-tinning, the selected agent was added to the blood sample before stannous ion. For pretinning, the agent was added to blood after the addition of stannous ion. In addition, a limited in vivo study in rats was conducted . For this study, the following agents were investigated for their effect on RBC labeling yield: sodium chromate, selenious acid, ferrous sulfate, digoxin and dimercaptosuccinic acid. Agents that reduced the RBC labeling yields in post-tinning in vitro procedures were ferric, ferrous, cupric, and stannic ions. Agents that reduced the labeling yields in both post- and pretinning in vitro procedures were dimercaptosuccinic acid, dimercaprol, edetic acid, methylene blue, chromate and selenite ions. Glucose and cysteine had no effect on the labeling yields of fresh or stored blood. Glutathione and inosine increased the labeling yields of stored blood. A high, but non-lethal concentration of cyanide ion did not alter the red blood cell labeling yield. When used at a ten fold increased concentration, it caused a reduction in the yields for both post- and pretinning procedures.
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