Sterol uptake in Saccharomyces cerevisiae

Graduate Thesis Or Dissertation

Public Deposited

Citeable URL: https://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/rj430805q

Descriptions

Attribute Name	Values
Creator	Salerno, Laurel F.
Abstract	An assay is described to measure sterol uptake in Saccharomyces cerevisiae. This assay involves specific binding of radioactively labelled sterol to actively growing yeast cells. It was found that uptake does not occur in stationary cultures and that some growth is necessary for FY3 , a sterol auxotroph, to take up exogenous sterol. Uptake is maximized at pH 5.5 and in the absence of Tween 80 in short term (4 hour) assays. The optimal temperature for uptake was found to be about 31° C. Most uptake studies were carried out at 28° C for convenience. The sterol uptake system exhibits Michaelis-Menten kinetics and shows a Km of 62.9 μg/ml cholesterol under the conditions described. The activation energy is 94000 cal/mole.
Resource Type	Masters Thesis
Date Available	2013-08-13T21:56:32+00:00
Date Issued	1982-12-15
Degree Level	Master's
Degree Name	Master of Science (M.S.)
Degree Field	Microbiology
Degree Grantor	Oregon State University
Commencement Year	1983
Advisor	Parks, Leo W.
Academic Affiliation	Microbiology
Non-Academic Affiliation	Oregon State University. Graduate School
Subject	Yeast Saccharomyces cerevisiae
Rights Statement	Copyright Not Evaluated
Publisher	Oregon State University
Peer Reviewed	No
Language	English [eng]
Digitization Specifications	File scanned at 300 ppi (Monochrome) using ScandAll PRO 1.8.1 on a Fi-6670 in PDF format. CVista PdfCompressor 4.0 was used for pdf compression and textual OCR.
Replaces	http://hdl.handle.net/1957/41699

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