Graduate Thesis Or Dissertation
 

Serological relationships of human T-mycoplasma isolates

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  • Ten T-mycoplasma strains isolated from the urine of male students with nongonococcal urethritis were compared in immunodiffusion, indirect hemagglutination (IHA), metabolic inhibition (MI), and immunoelectrophoretic tests using antigens prepared from these isolates and specific rabbit antisera. Immunodiffusion, MI, and IHA tests revealed complex antigenic interrelationships among the T-mycoplasmas, whereas immunoelectrophoretic analysis failed to resolve antigenic differences with the antigens and antisera utilized. The isolates were found to be antigenically heterogeneous by immunodiffusion, MI, and IHA tests, but all were related; the degree of heterogeneity was more pronounced in the MI tests than in the immunodiffusion and IHA tests. The results of the MI tests in this study confirmed the degree of serological heterogeneity observed by others in MI studies of T-mycoplasmas. Highly variable combinations of titers in both the MI and IHA tests precluded serotypic grouping of the isolates. The sensitivity of the immunodiffusion and IHA techniques were not as great as that of the MI technique. Heterologous anti-medium antibodies were not detected in the rabbit antisera by immunodiffusion tests. This is the first reported study to date in which T-mycoplasmas have been compared by immunodiffusion, IHA, and immunoelectrophoresis techniques. The results of this study suggest that immunodiffusion, MI, and IHA tests are useful in resolving antigenic differences of T-mycoplasmas; however, the usefulness of immunoelectrophoretic analysis for resolving antigenic differences of T-mycoplasmas is questionable.
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