Graduate Thesis Or Dissertation
 

Kinetic studies of the oxygen requirement of some amine oxidases

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  • Some kinetics of the oxygen requirement of amine oxidases have been investigated using bovine plasma monoamine oxidase (PAO) and pea seedling diamine oxidase (DAO). A system is described for studying enzyme reactions under controlled oxygen tension and in an O₂-free N₂ atmosphere. O₂ Km values were determined at two different levels of amine concentration for both PAO and DAO. The apparent O₂ Km values were found to vary with amine concentration and were in the order of 10⁻⁴ to 10⁻⁶ M depending on the enzyme and amine present in the reaction mixture. Ratios of moles of aldehyde product formed per mole of enzyme were determined under anaerobic conditions for PAO and DAO. The molar ratios were found to be approximately one mole of aldehyde per mole of enzyme in an O₂-free atmosphere for both enzymes. This suggests that the enzymes may produce a mole equivalent of aldehyde in the absence of oxygen.
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