Graduate Thesis Or Dissertation
 

The influence of (2-chloroethyl)-trimethylammonium chloride on the ribonucletic acid content of wheat (Triticum aestivum) leaves

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  • A study was made on the effect of CCC (2-chloroethyl)-trimethyl-ammonium chloride), a plant growth retardant, on the RNA (ribonucleic acid) content of wheat plants, It was found that the RNA content of wheat leaves generally decreased with increasing levels of CCC up to 1x10⁻⁵M. Thereafter, the RNA content again increased until the threshold of toxicity to wheat for this compound was reached. Very low levels, 1x10⁻⁸M., of CCC tended to slightly increase the RNA content of the same tissue. There was a qualitative difference in the RNA of treated plants as compared to the non-treated check. This qualitative difference was demonstrated by an alteration of the maximum absorbing wave length in the ultra-violet region of the spectrum. The non-treated material had the greatest optical density at 256.1 mμ, treatments of 1x10⁻⁸M. and 1x10⁻⁷M. CCC had a maximum optical density at 257 mμ, while the treatments of 1x10⁻⁶M. to 1x10⁻⁴M. CCC had an absorption peak at 257.75 mμ. The radio tracer data suggested there was less RNA-C¹⁴ present in the leaves of the plants treated at 1x10⁻⁵M. CCC compared to those of the non-treated in the period of thirty to thirty-three hours after the initial exposure to C¹⁴O₂. Thereafter, the labeled RNA appeared in nearly equal amounts in both tissues. The RNA-C¹⁴ level of the non-treated plants was almost constant in all of the harvests after the thirty-six hour period, whereas that of the treated material was quite variable. This data is interpreted as evidence of a cyclic synthesis and degradation of RNA, the periodicity of the cycle is a multiple of eight hours (the period between harvests). The CCC treated material also appeared to have a cycle. However, this cycle was out of phase with that of the normal plant. The cyclic nature of the RNA content was also determined spectrophotometrically. It was also apparent that the rate of RNA degradation was greatest in the plants treated with CCC. The RNA content data presented here were considered with that of the 2,4-D and other CCC work, the hypothesis that CCC increases the diffusible auxin content of treated tissue is supported.
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