Article
 

Inter-laboratory Validation of Bioaccessibility Testing for Metals

Public Deposited

Downloadable Content

Download PDF
https://ir.library.oregonstate.edu/concern/articles/qv33rz428

Descriptions

Attribute NameValues
Creator
Abstract
  • Bioelution assays are fast, simple alternatives to in vivo testing. In this study, the intra- and inter-laboratory variability in bioaccessibility data generated by bioelution tests were evaluated in synthetic fluids relevant to oral, inhalation, and dermal exposure. Using one defined protocol, five laboratories measured metal release from cobalt oxide, cobalt powder, copper concentrate, Inconel alloy, leaded brass alloy, and nickel sulfate hexahydrate. Standard deviations of repeatability (s[subscript r]) and reproducibility (s[subscript R]) were used to evaluate the intra- and inter-laboratory variability, respectively. Examination of the s[subscript R]:s[subscript r] ratios demonstrated that, while gastric and lysosomal fluids had reasonably good reproducibility, other fluids did not show as good concordance between laboratories. Relative standard deviation (RSD) analysis showed more favorable reproducibility outcomes for some data sets; overall results varied more between- than within-laboratories. RSD analysis of s[subscript r] showed good within-laboratory variability for all conditions except some metals in interstitial fluid. In general, these findings indicate that absolute bioaccessibility results in some biological fluids may vary between different laboratories. However, for most applications, measures of relative bioaccessibility are needed, diminishing the requirement for high inter-laboratory reproducibility in absolute metal releases. The inter-laboratory exercise suggests that the degrees of freedom within the protocol need to be addressed.
  • Keywords: Metals, Inter-laboratory validation, Read-across, Bioelution, Alloys, Classification, Bioaccessibility, UVCBs
  • Keywords: Metals, Inter-laboratory validation, Read-across, Bioelution, Alloys, Classification, Bioaccessibility, UVCBs
Resource Type
DOI
Date Available
Date Issued
Citation
  • Henderson, R. G., Verougstraete, V., Anderson, K., Arbildua, J. J., Brock, T. O., Brouwers, T., ... & Oller, A. R. (2014). Inter-laboratory validation of bioaccessibility testing for metals. Regulatory Toxicology and Pharmacology, 70(1), 170-181. doi:10.1016/j.yrtph.2014.06.021
Journal Title
Journal Volume
  • 70
Journal Issue/Number
  • 1
Rights Statement
Funding Statement (additional comments about funding)
  • The project was supported by the following: the Nickel Producers Environmental Research Association, Inc. (Durham, NC, USA), the Cobalt Development Institute (Guildford, Surrey UK), the International Zinc Association (Brussels, Belgium), the European Copper Institute (Brussels, Belgium), Eurometaux (Brussels, Belgium) and the Food Safety and Environmental Stewardship Program at OSU.
Publisher
Peer Reviewed
Language
Replaces

Relationships

Parents:

This work has no parents.

Items

Thumbnail Title Date Uploaded Visibility Actions
file details AndersonKimEnvironmentalMolecularToxicologyInterLlaboratoryValidationBioaccessibility.pdf 2017-07-25 Public Download