Undergraduate Thesis Or Project

 

Gene regulatory mechanisms of the lncRNA Fendrr Public Deposited

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https://ir.library.oregonstate.edu/concern/undergraduate_thesis_or_projects/h128ng533

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  • Embryonic development, where organs and major structures begin to develop, requires precise coordination of numerous gene regulatory pathways. Fendrr is an long non-coding RNA (lncRNA) that plays a role in regulating gene expression in mesodermal tissues during early embryonic development in mouse. The lateral plate mesoderm (LPM) is an earlier developed tissue that gives rise to lung tissue and ventricle structures such as the heart and body wall. Fendrr knockouts (FKOs) exhibit damaging changes to the tissues derived from LPM. Here, we compared FKOs to wild-type to measure genome-wide changes in gene expression to identify a possible regulatory targets for this transcript. We compared DNA accessibility through ATAC-seq, triplex site prediction, and RNA-seq data to determine the mechanisms of Fendrr’s interactions to regulate gene expression. FKOs expressed an upregulation in genes for lung tissue, equal amounts of regulation for LPM tissue, and downregulation for belly tissue. These results are consistent with the model that Fendrr suppresses expression through recruitment of Polycomb and the H3K27me3 chromatin mark to these genes.
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  • 2017-07-27 to 2021-06-09

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