Understanding the impact of orientation on gene expression of lux operon in pKN800 transformation into Escherichia coli DH5α

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Citeable URL: https://ir.library.oregonstate.edu/concern/defaults/n583z028r

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Attribute Name	Values
Creator	Seim, Darian T.
Abstract	Isolate and purify a pKN800 plasmid from Escherichia coli. Using restriction mapping determine the orientation of the Luciferase (lux) operon with respect to the plasmid backbone. Then transform the plasmid into E. coli strain DH5α to analyze the expression of the lux operon and the efficiency of transformation.
Resource Type	Other
Date Available	2017-05-01T21:15:50+00:00
Date Collected	2016-04-12/2016-04-18
Date Issued	2016
Non-Academic Affiliation	Oregon State University. Writing Intensive Curriculum
Rights Statement	Copyright Not Evaluated
Language	English [eng]
Replaces	http://hdl.handle.net/1957/60660

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