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Nonmuscle myosins II-B and Va are components of detergentresistant membrane skeletons derived from mouse forebrain

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dc.creator Ishmael, Jane E.
dc.creator Safic, Masa
dc.creator Amparan, David
dc.creator Vogel, Walter K.
dc.creator Pham, Tuyen
dc.creator Marley, Kevin
dc.creator Filtz, Theresa M.
dc.creator Maier, Claudia S.
dc.date.accessioned 2007-10-29T17:43:07Z
dc.date.available 2007-10-29T17:43:07Z
dc.date.issued 2007-10-29T17:43:07Z
dc.identifier.uri http://hdl.handle.net/1957/6771
dc.description.abstract Myosins are actin-based molecular motors that may have specialized trafficking and contractile functions in cytoskeletal compartments that lack microtubules. The postsynaptic excitatory synapse is one such specialization, yet little is known about the spatial organization of myosin motor proteins in the mature brain. We used a proteomics approach to determine if class II and class V myosin isoforms are associated with Triton X-100-resistant membranes isolated from mouse forebrain. Two nonmuscle myosin isoforms (II-B and Va), were identified as components of lipid raft fractions that also contained typical membrane skeletal proteins such as non-erythrocyte spectrins, actin, alpha-actinin-2 and tubulin subunits. Other raft-associated proteins included lipid raft markers, proteins involved in cell adhesion and membrane dynamics, receptors and channels including glutamate receptor subunits, scaffolding and regulatory proteins. Myosin II-B and Va were also present in standard postsynaptic density (PSD) fractions, however retention of myosin II-B was strongly influenced by ATP status. If homogenates were supplemented with ATP, myosin II-B could be extracted from PSD I whereas myosin Va and other postsynaptic proteins were resistant to extraction. In summary, both myosin isoforms are components of a raft-associated membrane skeleton and are likely detected in standard PSD fractions as a result of their intrinsic ability to form actomyosin. Myosin IIB, however, is loosely or more peripherally associated with the PSD than myosin Va, which appears to be a core PSD protein. en
dc.format.extent 2954650 bytes
dc.format.mimetype application/pdf
dc.language.iso en_US en
dc.subject lipid raft en
dc.subject mass spectrometry en
dc.subject actin cytoskeleton en
dc.subject proteomics en
dc.subject postsynaptic density en
dc.subject myosin II en
dc.subject myosin V en
dc.title Nonmuscle myosins II-B and Va are components of detergentresistant membrane skeletons derived from mouse forebrain en
dc.type Article en

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