Search Constraints

Start Over
Filtering by: Creator Haque, M. Remove constraint Creator: Haque, M. Academic Affiliation Biochemistry and Biophysics Remove constraint Academic Affiliation: Biochemistry and Biophysics

Search Results

  • Defining the Role of Tyrosine Nitration in Biology with Genetic Code Expansion

    Creator:
    Porter, Joseph J.
    Abstract:
    The buildup of reactive oxygen species (ROS) and reactive nitrogen and oxygen species (RNS) is known as oxidative stress. Oxidative stress results in a wide variety of modification to biological macromolecules including nucleic acids, lipids, and proteins. For at least 30 years it has been known that high levels of...
    Resource Type:
    Dissertation
    Full Text:
    synthetases (nitrotyrosyl-aaRS-tRNA) derived from the Methanocaldococcus janaschii (M. jannaschii) tyrosyl

  • Identifying the Enzymes Responsible for Reduction of Doxorubicin to its Cardiotoxic Metabolite Doxorubicinol using a Novel Immunoclearing Approach

    Creator:
    Breysse, Daniel H.
    Abstract:
    Doxorubicin is a widely used cancer therapeutic, but its effectiveness is limited by cardiotoxic side effects. Evidence suggests cardiotoxicity is due not to doxorubicin, but rather its metabolite, doxorubicinol. Identification of the enzymes responsible for doxorubicinol formation is important in developing strategies to prevent cardiotoxicity. In this study, the contributions...
    Resource Type:
    Masters Thesis
    Full Text:
    ) express roughly 10- fold higher levels of Cbr3 than wild type mice (Haque et al., 2010). Cbr3 was

  • Model systems for structural investigations into peroxiredoxin catalysis, conformation change, and inactivation

    Creator:
    Perkins, Arden
    Abstract:
    Peroxiredoxin (Prx) enzymes catalyze the reduction of hydrogen peroxide, peroxynitrous acid, and organic peroxides, and are extremely efficient peroxidases, with k[subscript cat]/K[subscript M] on the order of 10⁷ - 10⁸ M⁻¹ s⁻¹. Besides their role in oxidative stress defense, evidence has accumulated that some eukaryotes, including humans, use Prxs as...
    Resource Type:
    Dissertation
    Full Text:
    of 10 7 – 108 M-1 s-1. Besides their role in oxidative stress defense, evidence has accumulated

  • Peroxiredoxins in Parasites

    Creator:
    Gretes, Michael C., Poole, Leslie B., and Karplus, P. Andrew
    Abstract:
    Significance: Parasite survival and virulence relies on effective defenses against reactive oxygen and nitrogen species produced by the host immune system. Peroxiredoxins (Prxs) are ubiquitous enzymes now thought to be central to such defenses and, as such, have potential value as drug targets and vaccine antigens. Recent Advances: Plasmodial and...
    Resource Type:
    Article
    Full Text:
    efficiency (kcat/Km) values of 10 5 M - 1s - 1. Such reported catalytic rates, much lower than those of