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Microsporidiosis in zebrafish research facilities

Creator:: Sanders, Justin L., Watral, Virginia, and Kent, Michael L.
Abstract:: Pseudoloma neurophilia (Microsporidia) is the most common pathogen detected in zebrafish (Danio rerio) from research facilities. The parasite infects the central nervous system and muscle, and may be associated with emaciation and skeletal deformities. However, many fish exhibit subclinical infections. Another microsporidium, Pleistophora hyphessobryconis, has recently been detected in a...
Resource Type:: Article
Full Text:: 2 Justin L. Sanders*, Virginia Watral, Michael L. Kent 3 *Justin.Sanders@oregonstate.edu 4

Comparison of fixatives and fixation time for PCR detection of Mycobacterium in zebrafish Danio rerio

Creator:: Peterson, Tracy S., Kent, Michael L., Ferguson, Jayde A., Watral, Virginia G., and Whipps, Christopher M.
Abstract:: Mycobacteriosis is a common disease of laboratory zebrafish Danio rerio. Different infection patterns occur in zebrafish depending on mycobacterial species. Mycobacterium marinum and M. haemophilum produce virulent infections associated with high mortality, whereas M. chelonae is more widespread and is not associated with high mortality. Identification of mycobacterial infections to...
Resource Type:: Article
Full Text:: . Kent1,2, Jayde A. Ferguson4, Virginia G. Watral1, Christopher M. Whipps3 1Department of Microbiology

Expanding Diagnostic Assays for Pseudoloma neurophilia & Description of the Progression of Infection in Adult Zebrafish Populations

Creator:: Schuster, Corbin J.
Abstract:: Pseudoloma neurophilia is the most common pathogen reported in zebrafish (Danio rerio) research facilities and is an important threat to the zebrafish model. This microsporidian parasite can cause clinical disease, but more importantly is a causative agent of non-protocol induced variation in research. Studies utilizing infected zebrafish, could potentially confound...
Resource Type:: Dissertation
Full Text:: thank Virginia Watral, Lauren Norris, Elizanette Lopez, Taylor Kreul, Stepahnie Nervino, and Colleen

Final report : environmental stresses and skeletal deformities in fish from the Willamette River, Oregon, USA

Creator:: Villeneuve, Daniel L., Curtis, Lawrence R., Jenkins, Jeffrey J., Warner, Kara E., Tilton, Fred A., Kent, Michael L., Watral, Virginia G., Cunningham, Michael E., Markle, Douglas F., Sethajintanin, Doolalai, Krissanakriangkrai, Oraphin, Johnson, Eugene R., Grove, Robert Allan, Anderson, Kim A., Oregon State University. Department of Microbiology, Oregon State University. Department of Environmental and Molecular Toxicology, Oregon State University. Department of Fisheries and Wildlife, and Institute for Natural Resources
Abstract:: The Willamette River, one of only 14 American Heritage Rivers, flows through the most densely populated and agriculturally productive region of Oregon. Previous biological monitoring of Willamette River fish detected elevated frequencies of skeletal deformities in fish from certain areas of the lower (NP [NP], rivermile [RM] 26-55) and middle...
Resource Type:: Technical Report
Full Text:: A. Tilton†, Michael L. Kent‡, Virginia G. Watral‡, Michael E. Cunningham§, Douglas F. Markle

Controlling Important Pathogens in Zebrafish (Danio rerio) : Assessing Cryopreservation Survival of Bacteria and Parasites and Clinical Sensitivity of Mycobacterial qPCR Assays

Creator:: Norris, Lauren J.
Abstract:: Zebrafish (Danio rerio) are one of the most commonly used animal models in biomedical research. Zebrafish resource facilities, like the Zebrafish International Resource Center (ZIRC) in Eugene, Oregon, are the main providers and keepers of numerous zebrafish wild-type, mutant, and transgenic lines. Although ZIRC maintains live zebrafish at various life...
Resource Type:: Masters Thesis
Full Text:: AUTHORS Virginia Watral was the primary researcher for the 2014 Pseudoloma neurophilia

Detection and transmission of Mycobacterium marinum and Mycobacterium chelonae in zebrafish (Danio rerio)

Creator:: Peterson, Tracy Shawn
Abstract:: Mycobacteriosis is a common disease of laboratory zebrafish (Danio rerio). Different infection patterns occur in zebrafish depending on mycobacterial species. Mycobacterium marinum and M. haemophilum produce virulent infections associated with high mortality, whereas M. chelonae is more wide spread and not associated with high mortality. Identification of mycobacterial infections to...
Resource Type:: Dissertation
Full Text:: the development of this tumor. Virginia Watral assisted with injection of zebrafish and provided

Effects of Elevated Temperature on Mycobacterium chelonae Growth and Mycobacteriosis in Zebrafish (Danio rerio)

Creator:: Lopez, Elizanette V.
Abstract:: Utilization of zebrafish, Danio rerio, has steadily increased and its applications have expanded into numerous fields of science. Applying elevated temperatures (32°C to 37°C) to this organism has allowed researchers to conduct climate change, human cancer and infectious disease studies. Though zebrafish can be acclimated from the standard 28°C to...
Resource Type:: Masters Thesis
Full Text:: . Sanders and Kent, Virginia Watral and Lauren Norris. I thank each of them for their input and

Pseudoloma neurophilia : progression of infection and transmission characteristics of a microsporidian parasite in a model vertebrate, Danio rerio

Creator:: Sanders, Justin L.
Abstract:: The microsporidian parasite, Pseudoloma neurophilia, is the most commonly diagnosed infectious disease in laboratory populations of the zebrafish, Danio rerio. Infections by P. neurophilia are generally subclinical, however, they can become acute either incidentally or due to experimental immune suppression. Non-protocol induced variation can confound results in laboratory experiments using...
Resource Type:: Dissertation
Full Text:: Kent Laboratory, especially Virginia Watral, for putting up with me during these years and Dr. Trace

Erythrocytic inclusion body syndrome : salmonid stock susceptibility, secondary diseases, and vitamin therapy

Creator:: Shanks, Carol A.
Abstract:: Erythrocytic inclusion body syndrome (EIBS) was artificially established in selected stocks of juvenile fall and spring chinook salmon (Oncorhynchus tshawytscha), chum salmon (0. keta), coho salmon (0. kisutch), Atlantic salmon (Salmo salar), and rainbow trout (0. mykiss). Adult spring chinook salmon were also artificially infected with the EIBS virus. Adult...
Resource Type:: Masters Thesis
Full Text:: Gutenberger, Jiraporn Kasornchandra, Harriet Lorz, Michael Mauel, Jon Piganell, Glacier Tajwall, Virginia

Impacts of multispecies parasitism on coho salmon (Oncorhynchus kisutch) in Oregon

Creator:: Ferguson, Jayde A.
Abstract:: Diseases caused by parasites are recognized as significant sources of mortality in wild fish populations. I assessed the impacts of multispecies parasitism on a threatened stock of juvenile coho salmon (Oncorhynchus kisutch). A crucial prerequisite to this research was proper identification of parasites, which can be difficult for species lacking...
Resource Type:: Dissertation
Full Text:: rDNA sequence for Apophallus sp., Virginia Watral for general laboratory support, and Trace Peterson

ScholarsArchive@OSU

Microsporidiosis in zebrafish research facilities

Comparison of fixatives and fixation time for PCR detection of Mycobacterium in zebrafish Danio rerio

Expanding Diagnostic Assays for Pseudoloma neurophilia & Description of the Progression of Infection in Adult Zebrafish Populations

Final report : environmental stresses and skeletal deformities in fish from the Willamette River, Oregon, USA

Controlling Important Pathogens in Zebrafish (Danio rerio) : Assessing Cryopreservation Survival of Bacteria and Parasites and Clinical Sensitivity of Mycobacterial qPCR Assays

Detection and transmission of Mycobacterium marinum and Mycobacterium chelonae in zebrafish (Danio rerio)

Effects of Elevated Temperature on Mycobacterium chelonae Growth and Mycobacteriosis in Zebrafish (Danio rerio)

Pseudoloma neurophilia : progression of infection and transmission characteristics of a microsporidian parasite in a model vertebrate, Danio rerio

Erythrocytic inclusion body syndrome : salmonid stock susceptibility, secondary diseases, and vitamin therapy

Impacts of multispecies parasitism on coho salmon (Oncorhynchus kisutch) in Oregon

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