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Filtering by: Subject Poultry -- Diseases Advisor Andreasen, James R., jr.

Production of monoclonal antibodies against infectious laryngotracheitis virus of chickens and their use in an indirect immunofluorescenct diagnostic test

Creator:

Abbas, Ferhat, 1962-

Abstract:

Monoclonal antibodies were developed against USDA challenge strain of infectious laryngotracheitis virus (ILTV). Indirect immunofluorescence test was used to detect antibodies in supernatants of hybridomas. Hybridoma cells were developed by fusing Sp 2/0 myeloma cells with spleen cells obtained from mice immunized four times with partially purified USDA challenge strain...

Resource Type:

Masters Thesis

Full Text:

) is an acute disease of chickens characterized by signs of respiratory depression, gasping, and
Studies of immunological and molecular biological techniques with infectious laryngotracheitis virus of chickens

Creator:

Abbas, Ferhat, 1962-

Abstract:

Monoclonal antibodies (MCA) produced against infectious laryngotracheitis virus (ILTV) of chickens reacted in western blotting experiments with several different ILTV protein bands in the absence of tunicamycin which inhibits carbohydrate synthesis. Most of the MCA lost their reactivity in western blotting experiments when extracts of tunicamycin-treated ILTV CELC were used,...

Resource Type:

Dissertation

Full Text:

laryngotracheitis (ILT) is an acute disease of chickens caused by an alphaherpesvirus, infectious