PART I. The concentration of nickel in some soils may be
insufficient to meet the requirements of enzymes such as
urease in soybeans and hydrogenase in Rhizobium. Nickel
availability in soils was examined by measuring microbial
urease activity in soils which were treated with glucose
and urea. Supplemental nickel was...
The primary purpose of this investigation was to determine the
conditions necessary for the preparation of cell-free extracts of
nodule bacteroids capable of catalysing the reduction of acetylene to
ethylene and nitrogen to ammonia. Initial investigations were conducted
to determine whether intact soybean root nodules would reduce
acetylene to ethylene...
H₂-uptake positive strains (122 DES and SR) and H₂-uptake negative strains SR2 and SR3 of Rhizobium japonicum were examined for ribulosebisphosphate (RuBP) carboxylase and H₂-uptake activities during growth conditions which induced formation of the hydrogenase system. The rate of ¹⁴CO₂ uptake by hydrogenase-induced cells was about six times greater in...
A major objective of this investigation was to determine the
possible roles, if any, of poly-β-hydroxybutyrate and products of
its metabolic breakdown in symbiotic nitrogen fixation by nodule
bacteroids. Changes in poly -β- hydroxybutyrate content and in
activities of nitrogenase, β-hydroxybutyrate dehydrogenase, and
isocitrate lyase were studied under various conditions...