The first goal of this study was to evaluate the roles of different intracellular
proteolytic systems in accelerated skeletal muscle protein degradation. Total,
myofibrillar and non-myofibrillar proteolysis were determined in cultured L8/CID
muscle cells. Calpain proteolysis was assessed by over-expressed calpastatin inhibitory
domain (CD). Lysosomal proteolysis was determined in the...
Recombinant human calpain-5 was expressed in insect cells using a baculovirus system. The expressed calpain-5 was purified by both traditional chromatography and by affinity-column chromatography. Both methods yielded active protease. Calpain-5 displayed very limited hydrophobicity. This indicated that calpain-5 is not a membrane binding protein. Calpain-5 had pI of 8.3....
Glucocorticoids are growth-inhibiting steroids. They have been reported to reduce
muscle growth by reducing protein synthesis. However, their actions on muscle protein
degradation remain equivocal. Glucocorticoids have been reported to transiently increase
muscle protein degradation, to not affect this process and to reduce muscle protein
degradation. Reasons for these conflicting...