Six salmonid cell lines previously established in this laboratory,
CHSE 114 and CHSE 214 (chinook salmon), CSE 119 (coho salmon),
SSE 5 (sockeye salmon), STE 137 (steelhead trout) and RTH 149 (rainbow
trout), were partially characterized. Mycoplasmas were not
detected in any of the cell lines.
All cell lines exhibited...
Fish, asymptomatically infected with Enteric Redmouth
Bacterium (ERMB), were tested for their ability to transmit
bacteria to non-infected fish. Unstressed carrier fish
were unable to transmit enough bacteria to cause
epizootics or produce carrier fish. However, when the
ERMB carriers were stressed with heat, sufficient bacteria
were shed to infect...
Experiments were designed to more precisely evaluate the effects of water temperature on the progress of bacterial kidney disease ( BKD) in three species of salmonid fishes. Infections were produced by intraperitoneal injection of suspensions of the causative organism into fish held at seven temperatures ranging from 3.9 to 20.5°C....
Fish diseases and various parameters associated with disease
caused mortality of fish were monitored at the Oregon State University
Marine Science Center and at a private mariculture facility on
Yaquina Bay during a period of five years. Nearly all disease problems
observed were caused by Vibrio anguillarum and Vibrio spp....
Unfertilized ova from spring chinook salmon (Oncorhynchus
tshawytscha) were examined for the presence of classical salmonid
inmmunoglobulin. Immunodiffusion techniques in which rabbit anti-salmonid
immunoglobulin was reacted against an ova homogenate
and rabbit anti-ova homogenate was reacted against immune chinook
serum failed to detect classical-type antibodies within these eggs.
A lectin...
This study was undertaken to compile a comprehensive
review of the literature and to gain a better understanding
of the host-parasite relationship in corynebacterial kidney
disease of salmonid fishes. An indirect fluorescent antibody
technique was developed which allowed differentiation
of intra- and extracellular bacteria. This technique was
used in combination...
An additional layer, exterior to the outer membrane cellwall
layer, was found on Aeromonas salmonicida cells from aggregating
strains. Nonaggregating strains were found to lack this layer.
The nonaggregating strains were all avirulent (LD₅₀
greater than
1 X 10
⁸ CFU/fish) while the majority of the aggregating strains
were virulent...
Phenol-water and trichloroacetic acid extracts of Vibrio
anguillarum were both antigenic and immunogenic when injected
into coho salmon (Oncorhynchus kisutch). Cross-protection against
virulent Vibrio anguillarum was observed with whole-cell bacterins
and phenol-water extracts prepared from two serologically distinct
groups. Gel diffusion studies indicated that intact somatic antigens
were serotype specific....
A comparison of the agglutinating and precipatiating
antigens of Enteric Redmouth Bacterium (ERMB) was made.
There are two major and one minor agglutinating antigens
which describe two serotypes (I and II). Only serotype I
metabolizes sorbitol. A bacterin from serotype I cross
protected against a challenge of bacteria of serotype...
The distribution in Oregon of two viruses affecting salmonid
fishes was determined by examination of hatchery and feral fish.
Infectious pancreatic necrosis virus (IPNV) was isolated from fish
at the Oregon Department of Fish and Wildlife Research Laboratory,
Corvallis, Roaring River, Fort Klamath, and Wizard Falls Hatcheries.
Fish collected at...