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Insights into mammalian DNA replication from analysis of mutations affecting deoxyribonucleotide biosynthesis

Creator:: Mun, Byung-joo
Abstract:: Although the synthesis of DNA precursors is closely coordinated with DNA replication, it is still not clear whether deoxyribonucleoside triphosphates (dNTPs) influence DNA replication independently of their interactions with DNA polymerase catalytic sites. In an effort to understand the extent to which rate and fidelity of DNA replication are regulated...
Resource Type:: Dissertation
Full Text:: . Abstract approved: Redacted for Privacy Christopher K. Mathews Although the synthesis of DNA precursors

Mutagenic mechanisms associated with perturbations of DNA precursor biosynthesis in phage T4

Creator:: Ji, Jiuping
Abstract:: A crucial factor in determining the accuracy of DNA replication is maintenance of a balanced supply of deoxyribonucleoside triphosphates (dNTPs) at replication forks. Perturbation of dNTP biosynthesis can induce dNTP pool imbalance with deleterious genetic consequences, including increased mutagenesis, recombination, chromosomal abnormalities and cell death. Using the T4 bacteriophage system,...
Resource Type:: Dissertation
Full Text:: Perturbations of DNA Precursor Biosynthesis in Phage T4 Redacted for Privacy Abstract Approved: Christopher K

Mutagenic mechanisms associated with DNA cytosine methylation, DNA base sequence context and DNA precursor pool asymmetry

Creator:: Zhang, Xiaolin
Resource Type:: Dissertation
Full Text:: Asymmetry. Abstract approved: Christopher K. Mathews ABSTRACT A major goal of genetics is to

Enzyme associations in deoxyribonucleotide biosynthesis : anti-idiotypic antibodies as probes for direct protein-protein interactions

Creator:: Young, James Patrick
Abstract:: The ability to faithfully replicate DNA is dependent upon the maintenance and regulation of its precursors, the deoxyribonucleoside triphosphates. Enzymes encoded by the bacteriophage T4 have been widely used as models of biochemical processes. A body of evidence supports the concept that the bacteriophage T4 enzymes involved in deoxyribonucleotide biosynthesis...
Resource Type:: Dissertation
Full Text:: Christopher K. Mathews, Ph.D. The ability to faithfully replicate DNA is dependent upon the maintenance and

Modulation of deoxyribonucleoside triphosphate levels, DNA synthesis rates and fidelity in mammalian cells

Creator:: Martomo, Stella A.
Abstract:: Deoxyribonucleoside triphosphate (dNTP) concentrations measured in cells are not symmetric. dGTP almost always represents only 5-10% of the total dNTP pools in cells. In an in vitro replication system involving semiconservative replication from an SV 40 origin, the mutation frequency of an M13 phagemid replicated by human cell extracts in...
Resource Type:: Dissertation
Full Text:: . Abstract approved: Christopher K. Mathews Deoxyribonucleoside triphosphate (dNTP) concentrations measured

Precursors for mitochondrial DNA replication : metabolic sources and relations to mutagenesis and human diseases

Creator:: Song, Shiwei
Abstract:: It is well known that the mitochondrial genome has a much higher spontaneous mutation rate than the nuclear genome. mtDNA mutations have been identified in association with many diseases and aging. mtDNA replication continues throughout the cell cycle, even in post-mitotic cells. Therefore, a constant supply of nucleotides is required...
Resource Type:: Dissertation
Full Text:: Diseases Abstract approved:_ ___ Christopher K. Mathews It is well known that the mitochondrial genome

Organization of the T4 dNTP synthetase complex at DNA replication sites

Creator:: Kim, JuHyun
Abstract:: With respect to a multienzyme complex of deoxyribonucleoside triphosphate (dNTP) synthesis somehow juxtaposed with DNA replication sites, our laboratory has demonstrated the existence of a multienzyme complex in T4-infected E. coli, named the T4 dNTP synthetase complex, but the idea of direct linkage of dNTP synthesis to DNA replication and...
Resource Type:: Dissertation
Full Text:: of the T4 dNTP Synthetase Complex at DNA Replication Sites Abstract approved: Christopher K

Protein-protein interactions in the bacteriophage T4-coded dCTPase-dUTPase

Creator:: Ungermann, Christian
Resource Type:: Masters Thesis
Full Text:: like to thank Dr. Christopher K. Mathews for the opportunity to work in his laboratory and for his

DNA precursor compartmentation in mammalian cells : distribution and rates of equilibration between nucleus and cytoplasm

Creator:: Leeds, Janet M.
Abstract:: A rapid nuclear isolation technique was adapted in order to examine the question of DNA precursor compartmentation in mammalian cells. By using this method a reproducible proportion of the cellular nucleotides remained associated with the isolated nuclei. Examination, at several different cell densities, of exponentially growing HeLa cells showed that...
Resource Type:: Dissertation
Full Text:: encouragement of Dr. Christopher Mathews, and I wish to thank him for allowing me to work in his laboratory

Protein-protein interactions in the bacteriophage T4 dNTP synthetase complex

Creator:: Shen, Rongkun
Abstract:: The enzymes of dNTP synthesis in T4 infection associate to form a multienzyme complex, the T4 dNTP synthetase complex, facilitating the flow of metabolites en route to dNTPs, and their subsequent flow into DNA. Study of protein-protein interactions helps one to understand how the enzymes in the complex are organized...
Resource Type:: Dissertation
Full Text:: Synthetase Complex Abstract approved: Christopher K. Mathews The

ScholarsArchive@OSU

Insights into mammalian DNA replication from analysis of mutations affecting deoxyribonucleotide biosynthesis

Mutagenic mechanisms associated with perturbations of DNA precursor biosynthesis in phage T4

Mutagenic mechanisms associated with DNA cytosine methylation, DNA base sequence context and DNA precursor pool asymmetry

Enzyme associations in deoxyribonucleotide biosynthesis : anti-idiotypic antibodies as probes for direct protein-protein interactions

Modulation of deoxyribonucleoside triphosphate levels, DNA synthesis rates and fidelity in mammalian cells

Precursors for mitochondrial DNA replication : metabolic sources and relations to mutagenesis and human diseases

Organization of the T4 dNTP synthetase complex at DNA replication sites

Protein-protein interactions in the bacteriophage T4-coded dCTPase-dUTPase

DNA precursor compartmentation in mammalian cells : distribution and rates of equilibration between nucleus and cytoplasm

Protein-protein interactions in the bacteriophage T4 dNTP synthetase complex

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