Frankia from root nodules of nine different species of Ceanothus were
characterized. DNA was amplified directly from nodular material using the polymerase
chain reaction (PCR). The amplified region includes the 3' end of the 16S rRNA gene,
the intergenic spacer (IGS), and a large portion of the 23S rRNA gene....
The evolutionary relationship between Frankia and actinorhizal plants was evaluated
by reconstructing molecular phylogenetic trees from nifH, 16S rDNA, and rbcL
nucleotide sequences. Subgroupings in Frankia phylogenetic trees reconstructed from
nifH and from 16S rDNA sequences were consistent in terms of plant origins of Frankia
strains. Although the branching order...