Nitrification is a critical step in the global nitrogen cycle involving the biological oxidation of ammonia (NH₃) to nitrite (NO₂⁻ ) and then to nitrate (NO₃⁻). The first step in nitrification is carried out by NH₃-oxidizing bacteria (AOB) and archaea (AOA), and the second by NO₂⁻-oxidizing bacteria (NOB). In addition...
Prior to 2005, ammonia oxidation, the first step of nitrification, was thought mediated mainly by ammonia-oxidizing bacteria (AOB). However, the discovery of Thaumarchaeota carrying the genes coding for the ammonia monooxygenase (AMO) enzyme led to the discovery that ammonia-oxidizing archaea (AOA) also contribute to nitrification. Despite the uptick in studies...
Amendments and fertilizers applied to optimize crop production and improve soil health also supply inputs of soil organic matter (SOM) and nutrients that affect soil microorganisms. In the dairy-intensive Pacific Northwest, dairy manure is stored as slurry mixtures until conditions are appropriate for soil application. During storage, the manure begins...
Soil nitrogen exists largely as organic matter, including plant liter, dead animal matter, and microbial necromass. About 90% of soil organic nitrogen is proteinaceous material that is too large for plants and microorganisms to assimilate directly. Protein depolymerization therefore plays a critical role in mobilizing this organic source of nitrogen,...