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  • Factors affecting mitochondrial respiration in yeast

    Creator:
    Hough, Susan Carol
    Abstract:
    Two experimental approaches have been used to investigate the interaction of sterols with respiration in yeast. Cells were tested with an antifungal sterol, azasterol, and a mutant was obtained with an altered sterol composition. Polarographic measurements were done on mitochondria for both experiments. The effects of azasterol on mitochondrial respiration...
    Resource Type:
    Masters Thesis
    Full Text:
    the presence of substrate and ADP) by the polarographic slope of state 4 respiration (mitochondria in

  • Sterol 24(28) methylene reductase in Saccharomyces cerevisiae

    Creator:
    Neal, William Daniel
    Abstract:
    A cell free extract from Saccharomyces cerevisiae containing the enzyme that causes reduction of the 24(28)methylene group on the side chain of ergosterol has been studied. Optimal conditions for the 24(28)methylene reductase were obtained. The enzyme activity was increased in cells grown with ethanol as the substrate. The Km was...
    Resource Type:
    Masters Thesis
    Full Text:
    love and devotion TABLE OF CONTENTS I. INTRODUCTION Page 1 II. LITERATURE REVIEW 4 Early

  • Studies on sterol synthesis in yeast

    Creator:
    Starr, Patricia Rae
    Resource Type:
    Masters Thesis
    Full Text:
    $ohsol Oraduatc Conntttee H. Sohtlltug Redacted for Privacy Redacted for Privacy Redacted for Privacy

  • The distribution and influence of sterols in yeast membranes

    Creator:
    Bottema, Cynthia D. K.
    Abstract:
    Plasma membranes from Saccharomyces cerevisiae were prepared by a new procedure involving lyticase treatment of the yeast cells. The plasma membranes were right-side out, closed vesicles of uniform appearance with a sterol to phospholipid molar ratio of 0.365. The thermotropic behavior of these plasma membranes from wild-type yeast and from...
    Resource Type:
    Dissertation
    Full Text:
    components 23 F. Preparation of liposomes 23 G. Transmission electron microscopy 23 H. Enzyme assays 24 I

  • Defining roles for sterols in Saccharomyces cerevisiae : characterization of bulk membrane and high specificity sparking functions

    Creator:
    Rodriguez, Russell John
    Abstract:
    The ability of cholestanol to support growth of yeast sterol auxotrophs has been examined. Growth on this stanol was precluded unless minute quantities of ergosterol were available, a phenomenon designated sparking. The low levels of ergosterol were insufficient to support growth alone. The data indicate that cholestanol is fulfilling a...
    Resource Type:
    Dissertation
    Full Text:
    high school. TABLE OF CONTENTS INTRODUCTION LITERATURE REVIEW 1 4 MATERIALS AND METHODS 18

  • Physiological, biochemical, and genetic studies on the [delta]²⁴ sterol methyltransferase of Saccharomyces cerevisiae

    Creator:
    McCammon, Mark Thomas
    Abstract:
    The role of the yeast S-adenosylmethionine: Δ²⁴-sterol-C-methyltransferase (SCMT) in sterol metabolism was investigated. Structural analogs of S-adenosylhomocysteine were tested for inhibition of the SCMT enzyme. A wide inhibitory range by these compounds was observed, indicating which structural features of the parent compound are important for binding to the enzyme. The...
    Resource Type:
    Dissertation
    Full Text:
    . Measurement of Cellular SAM and SF 29 H. Sterol Analysis 29 I. Labelling of Lipids in Sterol Mutants 31 J