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Factors affecting mitochondrial respiration in yeast

Creator:: Hough, Susan Carol
Abstract:: Two experimental approaches have been used to investigate the interaction of sterols with respiration in yeast. Cells were tested with an antifungal sterol, azasterol, and a mutant was obtained with an altered sterol composition. Polarographic measurements were done on mitochondria for both experiments. The effects of azasterol on mitochondrial respiration...
Resource Type:: Masters Thesis
Full Text:: Abstract approved: Redacted for Privacy iLeo W. Parks Two experimental approaches have been used to

Sterol 24(28) methylene reductase in Saccharomyces cerevisiae

Creator:: Neal, William Daniel
Abstract:: A cell free extract from Saccharomyces cerevisiae containing the enzyme that causes reduction of the 24(28)methylene group on the side chain of ergosterol has been studied. Optimal conditions for the 24(28)methylene reductase were obtained. The enzyme activity was increased in cells grown with ethanol as the substrate. The Km was...
Resource Type:: Masters Thesis
Full Text:: (28)Methylene Reductase in Saccharomyces cerevisiae Abstract approved: Redacted for Privacy Leo W

Enzymatic studies on some methionine auxotrophs of yeast

Creator:: Pigg, C. JoAnne
Resource Type:: Masters Thesis
Full Text:: Sincere appreciation is exteded th Dr. Leo W. Parks for his direction and icouragement during the ccrae

The development of Phytophthora cactorum as a model system for the study of sterol metabolism

Creator:: Gonzales, Robert A.
Abstract:: Phytophthora cactorum lacks sterol synthesis capability. Nevertheless, it exhibits vegetative growth in the total absence of sterol. Supplementation of the growth medium with sterol, however, resulted in a three-fold increase in cell yield. This increase was shown by whole cell and cell free experiments to be the consequence of altered...
Resource Type:: Dissertation
Full Text:: Privacy 7 ABSTRACT Leo W. Parks Phytophthora cactorum lacks sterol synthesis capability. Neverthe

The distribution and influence of sterols in yeast membranes

Creator:: Bottema, Cynthia D. K.
Abstract:: Plasma membranes from Saccharomyces cerevisiae were prepared by a new procedure involving lyticase treatment of the yeast cells. The plasma membranes were right-side out, closed vesicles of uniform appearance with a sterol to phospholipid molar ratio of 0.365. The thermotropic behavior of these plasma membranes from wild-type yeast and from...
Resource Type:: Dissertation
Full Text:: Sterols in Yeast Membranes Abstract approved: Leo W. Parks Plasma membranes from Saccharomyces

S-adenosylmethionine : [triangle]²⁴-sterol methyltransferase and the regulation of ergosterol biosynthesis in Saccharomyces cerevisiae

Creator:: Bailey, Richard B.
Abstract:: The inhibition of S-aden.osylmethionine: Δ²⁴-sterol methyltransferase (EC 2.1.1.41) activity by endogenous cellular components has been studied in vitro. The principal inhibitors were Na⁺ and K⁺; Cs⁺, NH₄⁺ and Li⁺ were also shown to inhibit the reaction. The inhibition by potassium cation has been studied, and we have found that energy...
Resource Type:: Dissertation
Full Text:: TN SACCHAROMYCES CEREVISIAE Redacted for Privacy Abstract approved: r. Leo W. Parks The inhibition

Terminal methylation reactions in Saccharomyces cerevisiae

Creator:: Botsford, James Lawrence
Abstract:: A cell-free system for assessing the role of S-adenosyl-methionine in the methylation of homocysteine deriving the methyl group from serine has been developed. No role for S-adenosyl-methionine could be shown in the in vitro reaction The enzyme serine transhydroxymethylase has been studied in crude and partially purified cell extracts. Evidence...
Resource Type:: Dissertation
Full Text:: SACCHAROMYCES CERE VISIAE Abstract approved: Redacted for privacy ï Dr. Leo W. Parks A cell -free

The physiological effects of an antimycotic azasterol in Saccharomyces cerevisiae

Creator:: Hays, Phillip Roy
Resource Type:: Dissertation
Full Text:: Leo W. Parks The effects of 15-aza-24-methylene-8,14-homo- cholestadien-3S-ol on growth and sterol

Aspects of the regulation of methyl group formation in methionine biosynthesis by Saccharomyces cerevisiae

Creator:: Nakamura, Kenji Dennis
Resource Type:: Dissertation
Full Text:: CEREVISIAE Abstract approved: Redacted for Privacy (-7) Dr. Lei) W. Parks Serine transhydroxymethylase from

Amino acid metabolism in Acetobacter suboxydans

Creator:: Kerwar, Suresh Shivarau
Abstract:: Nutritional studies with Acetobacter suboxydans indicated that when isoleucine, lysine, methionine, serine and arginine were singly omitted from a complete amino acid mixture, no growth was obtained. However, the organism could grow on single amino acids like glutamic acid, histidine and proline. No growth was obtained with ammonium sulfate as...
Resource Type:: Dissertation
Full Text:: Pearson ACKNOWLEDGEMENT The author expresses his gratitude to Dr. Leo W. Parks. Dr. Vernon H

ScholarsArchive@OSU

Factors affecting mitochondrial respiration in yeast

Sterol 24(28) methylene reductase in Saccharomyces cerevisiae

Enzymatic studies on some methionine auxotrophs of yeast

The development of Phytophthora cactorum as a model system for the study of sterol metabolism

The distribution and influence of sterols in yeast membranes

S-adenosylmethionine : [triangle]²⁴-sterol methyltransferase and the regulation of ergosterol biosynthesis in Saccharomyces cerevisiae

Terminal methylation reactions in Saccharomyces cerevisiae

The physiological effects of an antimycotic azasterol in Saccharomyces cerevisiae

Aspects of the regulation of methyl group formation in methionine biosynthesis by Saccharomyces cerevisiae

Amino acid metabolism in Acetobacter suboxydans

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