Four experiments were conducted to determine oxytocin concen tration of bovine luteal tissue at different stages of the estrous cycle and to study the effects of prostaglandins F₂α (PGF₂α), E₂ (PGE₂), luteinizing hormone (LH), cycloheximide, colchicine and cytochalasin B on oxytocin synthesis and release in vitro. In experiment 1, luteal...
The lipid composition of plasma membranes of corpora
lutea was examined to determine whether changes in this
organelle occur during regression and early pregnancy in
the ewe. Forty ewes were assigned to be necropsied on
days 13 or 15 of the estrous cycle (D13-NP and D15-NP) or
pregnancy (D13-P and...
The role of the pituitary gland and uterus in regulating ovarian
function in the estradiol-treated gilt and ewe was investigated in two
experiments.
Pituitary gonadotropin content, ovarian follicular development and
corpus luteum function were studied following daily intramuscular
injection of seven gilts with 7 mg of 17β-estradiol from day 11...
Two experiments were conducted to determine whether in
ewes uterine concentrations of estrogen and progesterone
receptors are affected by the presence of a conceptus or by
the hormonal milieu associated with extremes in photoperiod
to which ewes are exposed.
In Exp.1, nine mature ewes were unilaterally
ovariectomized by removing an...
Two experiments were conducted to evaluate the effects of
gonadotropin releasing hormone (GnRH) on the function of the bovine
corpus luteum during the estrous cycle. In Experiment 1, 10 heifers
were assigned randomly into two groups with each heifer serving as
her own control. Heifers in Group I (n=5) were...
Experiments were conducted to characterize the nongenomic effects of
progesterone (P4) on binding of oxytocin (OT) to the ovine oxytocin
receptor (OTR) and signal transduction. The dose-response relationship of
P4 to OT binding to the OTR in endometrium was examined. Progesterone
interfered with the binding of OT to the OTR...
Two experiments were conducted to examine the effect of
a cyclopropenoid fatty acid on luteal cell function. In Exp.
1, 12 mature ewes were mated to a fertile ram, assigned to two
groups (n = 6/group) and laparotomized on day 18 of gestation.
Ewes with corpora lutea (CL) in both...