The interaction of skeletal muscle phosphofructokinase
with a variety of acidic proteins including calmodulin and
troponin C and with nucleic acids (RNA and DNA) is manifest
in a reversible, time-dependent loss of catalytic activity.
The inactivation is affected by a number of factors.
Substrates (fructose-6-phosphate and ATP), positive
effectors (fructose-2,6-bisphosphate,...
The consistent application of phosphatase inhibitors and a novel final purification step using a connected series of DE-51, DE-52, and DE-53 anion exchange chromatography columns facilitate the preparation of electrophoretically homogeneous sub-pupulations of rabbit muscle phosphofructokinase which differ in their catalytic properties and endogenous covalent phosphate contents. A band of...
This thesis examines the interconnection between the in vitro and in
vivo phosphorylation of rabbit muscle phosphofructokinase. The first goal
of the project was to show whether a novel site of rabbit muscle
phosphofructokinase that is subject to in vitro phosphorylation, serine 376,
may also become phosphorylated in vivo. Evidence...
The thermal denaturation of proteins has been extensively studied using several methods including differential scanning calorimetry (DSC). A custom-built optical system was used to study thermal effects on protein as an alternative method to DSC measurements. It was used to investigate the thermal stability of bovine serum albumin (BSA) with...