Index Catalog // ScholarsArchive@OSU

Ion pairing of nucleotides with surfactants for enhanced sensitivity in liquid matrix assisted secondary ion mass spectrometry

Creator:: Pavlovich, James Gilbert
Abstract:: In particle induced desorption-ionization mass spectrometry the strength of an analyte's signal under a given set of bombardment conditions is usually considered to be representative of the analytes relative surface activity. This rationale is generally used to explain differences in the technique's sensitivity between and within various classes of compound....
Resource Type:: Dissertation

Ion sputtering from organic liquid matrices bombarded by keV metal ions

Creator:: Yen, Ten-Yang
Resource Type:: Dissertation

Characterization of photochemically cross-linked protein-nucleic acid complexes by mass spectrometry

Creator:: Jensen, Ole Norregaard
Abstract:: A novel protocol for the study of protein-nucleic acid interactions is presented and demonstrated to be feasible. The protocol combines photochemical crosslinking techniques and mass spectrometric methods into a new strategy for identifying protein domains or amino acid residues that are in close contact with nucleic acid in protein-nucleic acid...
Resource Type:: Dissertation

Characterization of UV-crosslinked protein-nucleic acid interfaces by Maldi MS and ESI MS/MS

Creator:: Gafken, Philip R.
Resource Type:: Dissertation

Mass spectrometric analysis of UV-crosslinked protein-nucleic acid complexes

Creator:: Doneanu, Catalin E.
Abstract:: The DNA-binding domains of E. coli uracil-DNA glycosylase (Ung) and human replication protein A (hRPA) were studied using a general protocol developed in our laboratory for probing protein-DNA interactions. The procedure involves purification and mass spectrometric analysis of the nucleopeptide-products of a tryptically digested UV-crosslinked protein-nucleic acid complex. In the...
Resource Type:: Dissertation

Determination of fluorinated alkyl substances in aqueous systems

Creator:: Schultz, Melissa M.
Abstract:: Fluorinated alkyl substances, which can be persistent, toxic, and bioaccumulative, have been quantitated in many densely populated and remote regions, including in air, surface waters, groundwater, and biota; however, little is known about their transport or behavior in the environment. Wastewater effluent is one of the principal routes for introducing...
Resource Type:: Dissertation

Proteomic approach to the analysis of DNA-binding proteins using mass spectrometry

Creator:: Stapels, Martha Degen
Abstract:: In proteomic studies, separate experimental protocols have been necessary to identify proteins, determine their function, and predict their three-dimensional structure. In this study, a function-based separation of proteins was conceived to fractionate proteins prior to enzymatic digestion. In the initial demonstration of this technique, a DNA substrate was used to...
Resource Type:: Dissertation

Proteomic analyses of marine bacteria

Creator:: Sowell, Sarah M.
Abstract:: Proteins are the metabolic machines of the cell and as such, the study of proteins could illuminate the dominant biological activities that are occurring within cells and reveal how an organism interacts with its environment. Here, we used proteomic techniques to study the abundant marine bacterium SAR11 both as an...
Resource Type:: Dissertation

Chlordecone pretreatment promoted subcellular distribution of scavenger receptor class B type II to murine hepatic microsomes ; mass spectrometry detected hepatic soluble cholesterol binding proteins and comparison of protein iTRAQ ratios using ESI QTOF and MALDI TOF/TOF

Creator:: Scheri, Richard C.
Abstract:: Chlordecone belongs to the class of persistent organochlorine pesticides that are remarkably resistant to environmental degradation. Even though their use was banned in the United States in 1978, these compounds can still be detected in both humans and wildlife throughout the world. Previous work has shown that the pretreatment of...
Resource Type:: Dissertation

Gas-phase fragmentation chemistry of protonated peptide ions

Creator:: Bythell, Benjamin James
Abstract:: The fragmentation characteristics of protonated peptides have been investigated using tandem mass spectrometry (MS/MS) under various fragmentation regimes and detailed density functional theory (DFT) reaction pathway calculations. The DFT calculations predict novel salt-bridge stabilized transition structures as well as consecutive reactions occurring in proton-bound dimers for some of the ions...
Resource Type:: Dissertation

ScholarsArchive@OSU

Ion pairing of nucleotides with surfactants for enhanced sensitivity in liquid matrix assisted secondary ion mass spectrometry

Ion sputtering from organic liquid matrices bombarded by keV metal ions

Characterization of photochemically cross-linked protein-nucleic acid complexes by mass spectrometry

Characterization of UV-crosslinked protein-nucleic acid interfaces by Maldi MS and ESI MS/MS

Mass spectrometric analysis of UV-crosslinked protein-nucleic acid complexes

Determination of fluorinated alkyl substances in aqueous systems

Proteomic approach to the analysis of DNA-binding proteins using mass spectrometry

Proteomic analyses of marine bacteria

Chlordecone pretreatment promoted subcellular distribution of scavenger receptor class B type II to murine hepatic microsomes ; mass spectrometry detected hepatic soluble cholesterol binding proteins and comparison of protein iTRAQ ratios using ESI QTOF and MALDI TOF/TOF

Gas-phase fragmentation chemistry of protonated peptide ions

Limit your search

Academic Affiliation

Advisor

Commencement Year

Committee Member

Creator

Date

Decade

Degree Field

Degree Level

Degree Name

File Format

Language

License

Non-Academic Affiliation

Peer Reviewed

Resource Type

Rights Statement

Subject

Search Constraints

Search Results

Limit your search