Doxorubicin is a widely used cancer therapeutic, but its effectiveness is limited by cardiotoxic side effects. Evidence suggests cardiotoxicity is due not to doxorubicin, but rather its metabolite, doxorubicinol. Identification of the enzymes responsible for doxorubicinol formation is important in developing strategies to prevent cardiotoxicity. In this study, the contributions...
Replication associated protein and enzyme activity levels increase as cells enter
S-phase of the cell cycle and diminish as cells leave S-phase. Accordingly, replication
associated functions decrease as myoblasts withdraw from the cell cycle to terminally
differentiate. In an effort to understand signals effecting growth associated expression
of genes, the...
In budding yeast, many of the genes that encode enzymes required for DNA precursor synthesis (MCB genes) are expressed under cell cycle control in late G1/S. The relationship between MCB gene expression, dNTP synthesis and DNA synthesis was investigated by using α factor-synchronized Saccharomyces cerevisiae. The levels of all four...
Reporter gene transactivation by human p53 is inhibited in budding yeast lacking the TRR1 gene encoding thioredoxin reductase. Thioredoxin reductase specifically catalyzes the NADPH-dependent reduction of thioredoxin. Thioredoxin provides a source of electrons for disulfide reduction in various cellular processes. Reduction of disulfides within the cell can be accomplished by...
Aerobic organisms have evolved many sensory mechanisms that allow
response to oxidants in the environment. One area of interest is the relationship
between the activity of the tumor suppressor protein p53 and the redox state of
thioredoxin. Human p53 activity is severely compromised in budding yeast lacking
thioredoxin reductase. Evidence...