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Characterization of Gene Expression in Transgenic Plants with Modified Floral Gene Expression

Creator:

Colby, Samantha

Abstract:

The release of transgenes into the environment by transgenic plant species is a significant ecological concern, and consequently a containment method for these genetically modified organisms is highly sought after. For poplar trees, an option for containment is to produce trees that do not flower and therefore cannot spread genetic...

Resource Type:

Research Paper and Presentation
Agrobacterium-mediated transformation of hybrid poplar (Populus alba x P. grandidentata) and analysis of foreign gene expression

Creator:

Howe, Glenn Thomas

Abstract:

A method for Agrobacterium-mediated transformation of hybrid poplar (Populus alba x P. grandidentata) suspension cultures and regeneration of transformed plants is described. The best protocol was one in which suspension cultures were inoculated with Agrobacterium tumefaciens to a density of 10⁷ cfu's/ml, cocultivated for 48 hours, plated to cellulose acetate...

Resource Type:

Dissertation