The aim of this research was to gain more understanding on how a linker histone (LH), H1⁰, and high mobility group protein, HMG1, interact with nucleosome. To determine the location of linker proteins, three specific positioning sequences were used to reconstitute core nucleosomes and the binding of H1 and HMG1...
By studying the linker histone/DNA complex in vitro, our goal was to
gain further insight into the interaction of these histones in chromatin structure,
which, in turn, helps us in better understanding critical biological processes such
as replication, transcription, and recombination. In direct competition
experiments, we have confirmed previous results...