A kinetics-based enzyme-linked immunosorbent assay (KELA) for detection of serum IgG antibody against bovine respiratory syncytial virus (BRSV) was developed and optimized. Optimization factors tested included brand of microELISA plate, length of incubation periods at various temperatures, type of conjugate, purified versus precipitated antigen preparations, type of dilution buffer as...
Investigators have been studying an aggregate
containing enzymes of deoxyribonucleoside triphosphate
biosynthesis in T4-phage infected bacteria. They suggest
that it behaves as an organized complex, efficiently
channeling DNA precursors to the replication apparatus.
Previous work has suggested that about 10 enzyme
activities remain associated through several fractionation
steps. This multi-enzyme...
5'-Deoxy-5'-methylthioadenosine phosphorylase catalyzes
the phosphorolytic cleavage of 5'-deoxy-5'-methylthioadenosine
to form 5'-deoxy-5'-methylthioribose-1-phosphate and
adenine in mammalian cells and plays an important role in
methionine and purine salvage. The enzyme is abundant in
normal tissues and in cell lines derived from normal cells.
However, several malignant tissues and cell lines have been...
Three commercial sausage starter cultures were grown in
an internal-pH-controlled medium (PHASE 4) and in a soluble
phosphate-buffered medium. The cultures were tested by
comparing viable counts before and after freezing at -40 C
or freeze-drying and subsequent storage for 3 months in the
frozen or dried state. Strains varied...
The Baculoviridae is a family of DNA viruses which are obligate
pathogens for a variety of insects. Baculovirus strains came to the
attention of researchers because they provided a nonchemical
alternative for the biological control of insect pests of agriculture
and forestry (Ignoffo, 1968); however, recently they have been studied...