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Filtering by: Creator Townsend, M. Shaun Remove constraint Creator: Townsend, M. Shaun Committee Member Mahmud, Taifo Remove constraint Committee Member: Mahmud, Taifo

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  • Formation and modification of enduracididine, a nonproteinogenic amino acid

    Creator:
    Tan, Ying
    Abstract:
    Molecular genetic and enzymological techniques have been employed to study antibiotic biosynthesis. In this thesis, we studied the formation and modification of the nonproteinogenic amino acid enduracididine (End), which exists in two important antibiotics, mannopeptimycins (MPPs) and enduracidin. Sequence analysis of the MPP gene cluster revealed that the product of...
    Resource Type:
    Masters Thesis
    Full Text:
    ,VA, 2004 July 24.Available at http://www.idsociety.org. 2. Butler, M. S.; Buss A. D. Natural

  • Activation, incorporation and modification of capreomycidine during viomycin biosynthesis

    Creator:
    Fei, Xiaobo
    Abstract:
    Molecular genetic and enzymological techniques have been employed to study antibiotic biosynthesis. The nonproteinogenic amino acid capreomycidine is the signature residue found in the tuberactinomycin family of antitubercular peptide antibiotics and an important element of the pharmacophore. Recombinant VioG, a single module peptide synthetase from the viomycin gene cluster cloned...
    Resource Type:
    Masters Thesis
    Full Text:
    , which modifies nucleotide C1409 of the 16S RNA and C1920 of the 23S RNA. M. tuberculosis can acquire

  • Advanced studies on cyclic amino acids in neurological signaling and peptide antibiotics

    Creator:
    Blanchard, David, L.
    Abstract:
    L-pipecolic acid (L-PA) is the higher homolog of proline. It occurs naturally in many organisms, including primates, as an intermediate in lysine degradation. The pathway by which lysine is converted into L-pipecolic acid employs the enzyme Lpipecolate oxidase (L-PO), and appears to be tissue specific to the central nervous system...
    Resource Type:
    Dissertation

  • Ascorbylation- the biological relevance of covalently bound ascorbic acid

    Creator:
    Kesinger, Nicholas G.
    Abstract:
    Ascorbic acid, or vitamin C, is well known as a co-factor for proline-hydroxylase and as an anti-oxidant. However, it is also capable of forming covalent bonds, particularly in the role of a nucleophile, henceforth termed 'ascorbylation'. The ascorbylation of electrophiles can occur under physiological conditions. Furthermore, there are a number...
    Resource Type:
    Dissertation
    Full Text:
    -acid obtained by LC-MS analysis of a 3 h incubation of AscACR in H218O. The ions with m/z 205 in

  • Investigation of unique marine environments for microbial natural products

    Creator:
    Thornburg, Christopher C.
    Abstract:
    Metagenomics has revealed that the marine microbial biosphere is immensely more diverse than originally considered, and is an almost untapped reservoir for the potential discovery of microbial natural products. Despite numerous advances in culturing, biosynthetic engineering and genomic-based screening efforts to uncover much of this diversity in relatively accessible environments,...
    Resource Type:
    Dissertation
    Full Text:
    , San Diego. Biological testing for cancer cell cytotoxicity was performed by Andrew M. Hau, Jeffrey

  • Functional analysis of the biosynthetic gene cluster of the antitumor agent cetoniacytone A

    Creator:
    Wu, Xiumei
    Abstract:
    A gene cluster responsible for the biosynthesis of the antitumor agent cetoniacytone A was identified in Actinomyces sp. strain Lu 9419, an endosymbiotic bacteria isolated from the intestines of the rose chafer beetle. The nucleotide sequence analysis of the 26 Kb DNA region revealed the presence of 17 complete ORFs,...
    Resource Type:
    Dissertation
    Full Text:
    . I would like to thank Dr. Patricia M. Flatt for her relentless help and her hands-on training on

  • Biologically active metabolites from marine microorganisms : methods to identify drug leads and chemical signals

    Creator:
    Vining, Oliver B.
    Abstract:
    Microbial natural products represent a massive repository of unique chemical scaffolds with corresponding diverse biological functions. However, in the past decade the development of natural products into new therapeutics has dwindled, in part due to the challenges posed by high rediscovery rates and low throughput associated with classical bioassay-guided fractionation...
    Resource Type:
    Dissertation
    Full Text:
    . Mitchell performed phylogenetic and morphological analysis of cultured strains. Dr. Amanda M. Fenner

  • Deciphering the Function of New Gonorrhea Vaccine Candidates in Cell Envelope Homeostasis

    Creator:
    Baarda, Benjamin I.
    Abstract:
    Neisseria gonorrhoeae is the sexually transmitted pathogen responsible for millions of cases of gonorrhea worldwide each year. Rapidly-spreading antibiotic resistance is diminishing the ability to effectively treat a disease with significant consequences to female and male reproductive health, as well as to neonatal well-being. A protective vaccine has the potential...
    Resource Type:
    Dissertation