Under defined cultural conditions, zygotes of the brown alga
Fucus distichus L. Powell divide synchronously to form two-celled
embryos at approximately 24 hours after fertilization. These two
cells differ from one another in gross morphology, ultrastructure,
cytochemistry, and developmental fate. Cytochemical staining and
autoradiography indicated that a sulfated polysaccharide was...
The lack of a suitable demembranated cell model has hindered the
study of the mechanisms responsible for the regulation of ciliary
waveform and coordination. In this study procedures for the
isolation of ciliated cells from the newt, Taricha granulosa, by
trypsin dissociation, and their subsequent demembranation with Triton
X-100 and...
By using immobilized trypsin, I have been able to
prepare well-defined, stable trypsinized nucleosomes. The
difficulties of lacking of control in the extent of
trypsinization, which were encountered in previous studies
with the use of free trypsin, have been eliminated. The
nucleosomes and oligonucleosomes prepared by immobilized
trypsin are suitable...
Characterization of the 5' flanking region of rainbow trout ki-ras gene was begun with the cloning and sequencing of this region by the inverse PCR technique and dideoxynucleotide chain termination method. In total, a nucleotide sequence of 1080 bp upstream from the first coding ATG was sequenced. Although this region...
The vascular system of Aplysia californica is a simple visceromotor system with three main arteries innervated by a small pool of identified neurons. I used this system to
study the functions of neuropeptide transmitters and their actions on neural and muscle tissues. A central finding of this study is that...