Cryopreservation nearly universally depends on the equilibration of cells and tissues with high concentrations of permeating chemicals known as cryoprotective agents, or CPAs. Despite their protective properties, CPAs can cause damage as a result of osmotically-driven cell volume changes, as well as chemical toxicity. In this study, we have used...
Ice-free cryopreservation, known as vitrification, is an appealing approach for banking of adherent cells and tissues because it prevents dissociation and morphological damage that may result from ice crystal formation. However, current vitrification methods are often limited by the cytotoxicity of the concentrated cryoprotective agent (CPA) solutions that are required...
BACKGROUND: Simple and effective cryopreservation of human oocytes would have
an enormous impact on the financial and ethical constraints of human assisted
reproduction. Recently, studies have demonstrated the potential for cryopreservation
in an ice-free glassy state by equilibrating oocytes with high concentrations of
cryoprotectants (CPAs) and rapidly cooling to liquid...