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Virus-Derived Gene Expression and RNA Interference Vector for Grapevine

Creator:: Kurth, Elizabeth G., Peremyslov, Valera V., Prokhnevsky, Alexey I., Kasschau, Kristin D., Miller, Marilyn, Carrington, James C., and Dolja, Valerian V.
Abstract:: The improvement of the agricultural and wine-making qualities of the grapevine (Vitis vinifera) is hampered by adherence to traditional varieties, the recalcitrance of this plant to genetic modifications, and public resistance to genetically modified organism (GMO) technologies. To address these challenges, we developed an RNA virus-based vector for the introduction...
Resource Type:: Article
Full Text:: ):6002. DOI: 10.1128/JVI.00436-12. J. Virol. Carrington and Valerian V. Dolja Prokhnevsky, Kristin D

Small RNA binding is a common strategy to suppress RNA silencing by several viral suppressors

Creator:: Lakatos, Lóránt, Csorba, Tibor, Pantaleo, Vitantonio, Chapman, Elisabeth J., Carrington, James C., Liu, Yu-Ping, Dolja, Valerian V., Calvino, Lourdes Fernández, López-Moya, Juan José, and Burgyán, József
Abstract:: RNA silencing is an evolutionarily conserved system that functions as an antiviral mechanism in higher plants and insects. To counteract RNA silencing, viruses express silencing suppressors that interfere with both siRNA- and microRNA-guided silencing pathways. We used comparative in vitro and in vivo approaches to analyse the molecular mechanism of...
Resource Type:: Article
Full Text:: Chapman2, James C Carrington2,3, Yu-Ping Liu2, Valerian V Dolja2,3, Lourdes Fernández Calvino4, Juan José

Formation of Complexes at Plasmodesmata for Potyvirus Intercellular Movement Is Mediated by the Viral Protein P3N-PIPO

Creator:: Wei, Taiyun, Zhang, Changwei, Hong, Jian, Xiong, Ruyi, Kasschau, Krisitin D., Zhou, Xueping, Carrington, James C., and Wang, Aiming
Abstract:: Intercellular transport of viruses through cytoplasmic connections, termed plasmodesmata (PD), is essential for systemic infection in plants by viruses. Previous genetic and ultrastructural data revealed that the potyvirus cyclindrical inclusion (CI) protein is directly involved in cell-to-cell movement, likely through the formation of conical structures anchored to and extended through...
Resource Type:: Article
Full Text:: the myosins XI-K and VIII-1 tails were kindly provided by Valerian V. Dolja (Oregon State University

Complementation of tobacco etch potyvirus mutants by active RNA polymerase expressed in transgenic cells

Creator:: Li, Xiao Hua and Carrington, James C.
Abstract:: A genetic complementation system was developed in which tobacco etch virus (TEV) polymerase (NIb)- expressing transgenic plants or protoplasts were inoculated with NIb-defective TEV mutants. A β-glucuronidase (GUS) reporter gene integrated into the genomes of parental and four mutant viruses was used to assay RNA amplification. Two mutants (termed VNN...
Resource Type:: Article
Full Text:: . & Zabel, P. (1984) Proc. Natl. Acad. Sci. USA 81, 1951-1955. 13. Dolja, V. V., McBride, H. J. & Carrington

Evidence that the Potyvirus P1 Proteinase Functions in trans as an Accessory Factor for Genome Amplification

Creator:: Verchot, Jeanmarie and Carrington, James C.
Abstract:: The tobacco etch potyvirus (TEV) polyprotein is proteolytically processed by three viral proteinases (NIa, HC-Pro, and P1). While the NIa and HC-Pro proteinases each provide multiple functions essential for viral infectivity, the role of the P1 proteinase beyond its autoproteolytic activity is understood poorly. To determine if P1 is necessary...
Resource Type:: Article
Full Text:: . Carrington, J. C., R. Haldeman, V. V. Dolja, and M. A. Restrepo-Hartwig. 1993. Internal cleavage and trans

Debilitation of Plant Potyvirus Infectivity by P1 Proteinase-Inactivating Mutations and Restoration by Second-Site Modifications

Creator:: Verchot, Jeanmarie and Carrington, James C.
Abstract:: Tobacco etch virus (TEV) encodes three proteinases that catalyze processing of the genome-encoded polyprotein. The P1 proteinase originates from the N terminus of the polyprotein and catalyzes proteolysis between itself and the helper component proteinase (HC-Pro). Mutations resulting in substitution of a single amino acid, small insertions, or deletions were...
Resource Type:: Article
Full Text:: complete processing. EMBO J. 9:1347–1353. 10. Carrington, J. C., R. Haldeman, V. V. Dolja, and M. A

Analysis of the VPg-Proteinase (NIa) Encoded by Tobacco Etch Potyvirus: Effects of Mutations on Subcellular Transport, Proteolytic Processing, and Genome Amplification

Creator:: Schadd, Mary C., Haldeman-Chahill, Ruth, Cronin, Stephen, and Carrington, James C.
Abstract:: A mutational analysis was conducted to investigate the functions of the tobacco etch potyvirus VPgproteinase (NIa) protein in vivo. The NIa N-terminal domain contains the VPg attachment site, whereas the C-terminal domain contains a picornavirus 3C-like proteinase. Cleavage at an internal site separating the two domains occurs in a subset...
Resource Type:: Article
Full Text:: s in fe ct ed by pa re nt al T E V -G U S or th e N L S do ub le m ut an ts .P ro to pl as ts w er e

RTM3, Which Controls Long-Distance Movement of Potyviruses, Is a Member of a New Plant Gene Family Encoding a Meprin and TRAF Homology Domain-Containing Protein

Creator:: Cosson, Patrick, Sofer, Luc, Hien Le, Quang, Léger, Valérie, Schurdi-Levraud, Valérie, Whitham, Steven A., Yamamoto, Miki L., Gopalan, Suresh, Le Gall, Olivier, Chandresse, Thierry, Carrington, James C., and Revers, Frédéric
Abstract:: Restriction of long-distance movement of several potyviruses in Arabidopsis (Arabidopsis thaliana) is controlled by at least three dominant restricted TEV movement (RTM) genes, named RTM1, RTM2, and RTM3. RTM1 encodes a protein belonging to the jacalin family, and RTM2 encodes a protein that has similarities to small heat shock proteins....
Resource Type:: Article
Full Text:: ) and TEV-GUS (Dolja et al., 1992) were used for the inoculation experiments. Inoculation of

Garcia-RuizHernanBotanyPlantPathologyRolesProgramming.pdf

Creator:: Garcia-Ruiz, Hernan, Carbonell, Alberto, Hoyer, J. Steen, Fahlgren, Noah, Takeda, Atsushi, Giampetruzzi, Annalisa, Lowery, Nicholas, Carrington, James C., and et al.
Abstract:: In eukaryotes, ARGONAUTE proteins (AGOs) associate with microRNAs (miRNAs), short interfering RNAs (siRNAs), and other classes of small RNAs to regulate target RNA or target loci. Viral infection in plants induces a potent and highly specific antiviral RNA silencing response characterized by the formation of virus-derived siRNAs. Arabidopsis thaliana has...
Full Text:: ago1–27 ago2–1 ago10–5 triple mutants by TuM- V-AS9-GFP was similar to that of dcl2–1 dcl3–1 dcl4–2

ScholarsArchive@OSU

Virus-Derived Gene Expression and RNA Interference Vector for Grapevine

Small RNA binding is a common strategy to suppress RNA silencing by several viral suppressors

Formation of Complexes at Plasmodesmata for Potyvirus Intercellular Movement Is Mediated by the Viral Protein P3N-PIPO

Complementation of tobacco etch potyvirus mutants by active RNA polymerase expressed in transgenic cells

Evidence that the Potyvirus P1 Proteinase Functions in trans as an Accessory Factor for Genome Amplification

Debilitation of Plant Potyvirus Infectivity by P1 Proteinase-Inactivating Mutations and Restoration by Second-Site Modifications

Analysis of the VPg-Proteinase (NIa) Encoded by Tobacco Etch Potyvirus: Effects of Mutations on Subcellular Transport, Proteolytic Processing, and Genome Amplification

RTM3, Which Controls Long-Distance Movement of Potyviruses, Is a Member of a New Plant Gene Family Encoding a Meprin and TRAF Homology Domain-Containing Protein

Garcia-RuizHernanBotanyPlantPathologyRolesProgramming.pdf

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