Index Catalog // ScholarsArchive@OSU

Evidence that the Potyvirus P1 Proteinase Functions in trans as an Accessory Factor for Genome Amplification

Creator:: Verchot, Jeanmarie and Carrington, James C.
Abstract:: The tobacco etch potyvirus (TEV) polyprotein is proteolytically processed by three viral proteinases (NIa, HC-Pro, and P1). While the NIa and HC-Pro proteinases each provide multiple functions essential for viral infectivity, the role of the P1 proteinase beyond its autoproteolytic activity is understood poorly. To determine if P1 is necessary...
Resource Type:: Article
Full Text:: transgenic (Tran) plants inoculated with TEV-GUS or the S256A mutant. Lanes: 1, DNA size standards; 2 to 10

Functional Analysis of Three Arabidopsis ARGONAUTES Using Slicer-Defective Mutants

Creator:: Carbonell, Alberto, Fahlgren, Noah, Garcia-Ruiz, Hernan, Gilbert, Kerrigan B., Montgomery, Taiowa A., Nguyen, Tammy, Cuperus, Josh T., and Carrington, James C.
Abstract:: In RNA-directed silencing pathways, ternary complexes result from small RNA-guided ARGONAUTE (AGO) associating with target transcripts. Target transcripts are often silenced through direct cleavage (slicing), destabilization through slicer-independent turnover mechanisms, and translational repression. Here, wild-type and active-site defective forms of several Arabidopsis thaliana AGO proteins involved in posttranscriptional silencing were...
Resource Type:: Article
Full Text:: from several known AGO1-miRNA–targeted tran- scripts, such as SCARECROW-LIKE6 (SCL6), AUXIN RESPONSE

Genetic and Functional Diversification of Small RNA Pathways in Plants

Creator:: Xie, Zhixin, Johansen, Lisa K., Gustafan, Adam M., Kasschau, Kristin D., Lellis, Andrew D., Zilberman, Daniel, Jacobsen, Steven E., and Carrington, James C.
Abstract:: Multicellular eukaryotes produce small RNA molecules (approximately 21–24 nucleotides) of two general types, microRNA (miRNA) and short interfering RNA (siRNA). They collectively function as sequence-specific guides to silence or regulate genes, transposons, and viruses and to modify chromatin and genome structure. Formation or activity of small RNAs requires factors belonging...
Resource Type:: Article
Full Text:: Small RNA Pathways in Plants Zhixin Xie 1[, Lisa K. Johansen 1[, Adam M. Gustafson 1 , Kristin D

Autocatalytic Processing of the Potyvirus Helper Component Proteinase in Escherichia coli and In Vitro

Creator:: Carrington, James C., Freed, Deon D., and Sanders, Tatia C.
Abstract:: The virus-encoded proteins of tobacco etch virus (TEV), a plant potyvirus, arise by proteolytic processing of a large polyprotein precursor. The TEV genome codes for two proteinases, a 49-kilodalton proteinase and helper component proteinase (HC-Pro), which cleave the polyprotein at specific sites. The only known cleavage event catalyzed by HC-Pro...
Resource Type:: Article
Full Text:: protease. J. Virol. 61:2540-2548. 11. Domier, L. L., K. M. Franklin, M. Shahabuddin, G. M. Hell- mann, J

Small RNAs serve as a genetic buffer against genomic shock in Arabidopsis interspecific hybrids and allopolyploids

Creator:: Ha, Misook, Lu, Jie, Ramachandran, Vanitharani, Kasschau, Krisitin D., Chapman, Elisabeth J., Carrington, James C., Chen, Xuemei, Wang, Xue-Jei, and Chen, Z. Jeffrey
Abstract:: Small RNAs, including microRNAs (miRNAs), small interfering RNAs (siRNAs), and trans-acting siRNAs (tasiRNAs), control gene expression and epigenetic regulation. Although the roles of miRNAs and siRNAs have been extensively studied, their expression diversity and evolution in closely related species and interspecific hybrids are poorly understood. Here, we show comprehensive analyses...
Resource Type:: Article
Full Text:: RNAs (F1) Small RNAs (F7) Small RNAs (As) S m al lR N A s (k bp /1 00 -k bp ) Gene density

Analysis of the VPg-Proteinase (NIa) Encoded by Tobacco Etch Potyvirus: Effects of Mutations on Subcellular Transport, Proteolytic Processing, and Genome Amplification

Creator:: Schadd, Mary C., Haldeman-Chahill, Ruth, Cronin, Stephen, and Carrington, James C.
Abstract:: A mutational analysis was conducted to investigate the functions of the tobacco etch potyvirus VPgproteinase (NIa) protein in vivo. The NIa N-terminal domain contains the VPg attachment site, whereas the C-terminal domain contains a picornavirus 3C-like proteinase. Cleavage at an internal site separating the two domains occurs in a subset...
Resource Type:: Article
Full Text:: pTL7SN-derived plasmids. Tran- scripts were recovered from the reactions by precipitation in the presence

Complementation of tobacco etch potyvirus mutants by active RNA polymerase expressed in transgenic cells

Creator:: Li, Xiao Hua and Carrington, James C.
Abstract:: A genetic complementation system was developed in which tobacco etch virus (TEV) polymerase (NIb)- expressing transgenic plants or protoplasts were inoculated with NIb-defective TEV mutants. A β-glucuronidase (GUS) reporter gene integrated into the genomes of parental and four mutant viruses was used to assay RNA amplification. Two mutants (termed VNN...
Resource Type:: Article
Full Text:: . 67, 6995-7000. 18. Dolja, V. V., Herndon, K. L., Pirone, T. P. & Carrington, J. C. (1993) J. Virol

Identification and Characterization of Human Cytomegalovirus-Encoded MicroRNAs

Creator:: Grey, Finn, Antoniewicz, Andy, Allen, Edwards, Saugstad, Julie, McShea, Andy, Carrington, James C., and Nelson, Jay
Abstract:: MicroRNAs (miRNAs) are an extensive class of noncoding genes that regulate gene expression through posttranscriptional repression. Given the potential for large viral genomes to encode these transcripts, we examined the human cytomegalovirus AD169 genome for miRNAs using a bioinformatics approach. We identified 406 potential stem-loops, of which 110 were conserved...
Resource Type:: Article
Full Text:: each of the 406 candidate SLF-derived HCMV stem-loop tran- scripts was compared with the CCMV genome

Cap-Independent Enhancement of Translation by a Plant Potyvirus 5' Nontranslated Region

Creator:: Carrington, James C. and Freed, Deon D.
Abstract:: The RNA genome of tobacco etch virus (TEV), a plant potyvirus, functions as an mRNA for synthesis of a 346-kilodalton polyprotein that undergoes extensive proteolytic processing. The RNA lacks a normal 5' cap structure at its terminus, which suggests that the mechanism of translational initiation differs from that of a...
Resource Type:: Article
Full Text:: . 1). The 35S promoter, GUS-encoding sequence, and tran- scriptional terminator in pRT-GUS and pRTL

Debilitation of Plant Potyvirus Infectivity by P1 Proteinase-Inactivating Mutations and Restoration by Second-Site Modifications

Creator:: Verchot, Jeanmarie and Carrington, James C.
Abstract:: Tobacco etch virus (TEV) encodes three proteinases that catalyze processing of the genome-encoded polyprotein. The P1 proteinase originates from the N terminus of the polyprotein and catalyzes proteolysis between itself and the helper component proteinase (HC-Pro). Mutations resulting in substitution of a single amino acid, small insertions, or deletions were...
Resource Type:: Article
Full Text:: before (18) but with the exclusion of the CsCl gradient step. Viral RNA was isolated by proteinase K

ScholarsArchive@OSU

Evidence that the Potyvirus P1 Proteinase Functions in trans as an Accessory Factor for Genome Amplification

Functional Analysis of Three Arabidopsis ARGONAUTES Using Slicer-Defective Mutants

Genetic and Functional Diversification of Small RNA Pathways in Plants

Autocatalytic Processing of the Potyvirus Helper Component Proteinase in Escherichia coli and In Vitro

Small RNAs serve as a genetic buffer against genomic shock in Arabidopsis interspecific hybrids and allopolyploids

Analysis of the VPg-Proteinase (NIa) Encoded by Tobacco Etch Potyvirus: Effects of Mutations on Subcellular Transport, Proteolytic Processing, and Genome Amplification

Complementation of tobacco etch potyvirus mutants by active RNA polymerase expressed in transgenic cells

Identification and Characterization of Human Cytomegalovirus-Encoded MicroRNAs

Cap-Independent Enhancement of Translation by a Plant Potyvirus 5' Nontranslated Region

Debilitation of Plant Potyvirus Infectivity by P1 Proteinase-Inactivating Mutations and Restoration by Second-Site Modifications

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